Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp

PloS One
Dandan MaBuling Wu

Abstract

Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated that CDPSCs had a higher proliferative and stronger osteogenic differentiation potential than DPSCs. However, the molecular mechanisms responsible for the biological differences between DPSCs and CDPSCs are poorly understood. The aim of this study was to define the molecular features of DPSCs and CDPSCs by comparing the proteomic profiles using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that there were 18 protein spots differentially expressed between DPSCs and CDPSCs in a narrow pH range of 4 to 7. These differently expressed proteins are mostly involved in the regulation of cell proliferation, differentiation, cell cytoskeleton and motility. In addition, our results suggested that CDPSCs had a higher expression of antioxidative proteins that might protect CDPSCs from oxid...Continue Reading

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Citations

Mar 1, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·P Gomes-AlvesP M Alves
Oct 30, 2016·Journal of Cellular Physiology·Jie LiJinlin Song
Jan 6, 2018·International Journal of Molecular Sciences·Vasiliki TarasliaEma Anastasiadou
Apr 15, 2018·Journal of Cellular and Molecular Medicine·Dandan MaBuling Wu
Feb 11, 2020·Cell Biology International·Hanbing LiangChen Zhang
Nov 13, 2020·World Journal of Stem Cells·Jagadish HosmaniHarish C Chandramoorthy
May 13, 2021·Clinical Oral Investigations·Yaghoup Feridouni KhamanehReinhard E Friedrich
Jul 3, 2021·International Journal of Molecular Sciences·Sarah Hani ShoushrahEdda Tobiasch

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Methods Mentioned

BETA
proteomic profiling
electrophoresis
scanning electron microscopy

Software Mentioned

SPSS Statistics
DeCyder
Mascot

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