Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp

PloS One
Dandan MaBuling Wu


Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated that CDPSCs had a higher proliferative and stronger osteogenic differentiation potential than DPSCs. However, the molecular mechanisms responsible for the biological differences between DPSCs and CDPSCs are poorly understood. The aim of this study was to define the molecular features of DPSCs and CDPSCs by comparing the proteomic profiles using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that there were 18 protein spots differentially expressed between DPSCs and CDPSCs in a narrow pH range of 4 to 7. These differently expressed proteins are mostly involved in the regulation of cell proliferation, differentiation, cell cytoskeleton and motility. In addition, our results suggested that CDPSCs had a higher expression of antioxidative proteins that might protect CDPSCs from oxid...Continue Reading


Oct 28, 1994·Biochemical and Biophysical Research Communications·P H Krone, J B Sass
Nov 10, 2000·Developmental Biology·K MochidaA L Kierszenbaum
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Sep 14, 2001·Biochemical and Biophysical Research Communications·A KitamuraM Imagawa
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Sep 18, 2002·Journal of Dermatological Science·Xiu-Kun SunXue-Jun Zhu
Dec 17, 2002·Vitamins and Hormones·Hiroshi Kubota
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
May 3, 2003·Biochemical and Biophysical Research Communications·Kei ShimizuKiyomi Kikugawa
Aug 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·M Charlotte OlssonRichard L Moss
Sep 15, 2004·Trends in Biochemical Sciences·Martin A Wear, John A Cooper
Sep 16, 2004·Journal of Cellular Biochemistry·Camelia Iancu Rubin, George F Atweh
Jan 22, 2005·Journal of the American Society of Nephrology : JASN·Chih-Chang WeiJohn S D Chan
May 14, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·C MorsczeckK H Hoffmann
Jul 15, 2005·Molecular Reproduction and Development·Eugene RivkinAbraham L Kierszenbaum
Jun 2, 2006·The Journal of Biological Chemistry·Sambanthamurthy SoundarRoberta F Colman
Jun 24, 2006·Oral Diseases·S IvanovskiP M Bartold
Nov 1, 2006·Journal of Cell Science·Nuria Pujol-CarrionMaria Angeles de la Torre-Ruiz
Dec 22, 2006·PloS One·Wataru SonoyamaSongtao Shi
Jul 20, 2007·Journal of Cell Science·Kwang S SuhStuart H Yuspa
Dec 28, 2007·Experimental & Molecular Medicine·Moon-Young ChoiSung Won Kwon
Jun 12, 2008·International Review of Cell and Molecular Biology·John A Cooper, David Sept
Aug 2, 2008·European Cells & Materials·G BluteauT A Mitsiadis
Oct 22, 2008·Journal of Molecular and Cellular Cardiology·Hyeseon ChaWoo Jin Park

❮ Previous
Next ❯


Mar 1, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·P Gomes-AlvesP M Alves
Oct 30, 2016·Journal of Cellular Physiology·Jie LiJinlin Song
Jan 6, 2018·International Journal of Molecular Sciences·Vasiliki TarasliaEma Anastasiadou
Apr 15, 2018·Journal of Cellular and Molecular Medicine·Dandan MaBuling Wu
Feb 11, 2020·Cell Biology International·Hanbing LiangChen Zhang
Nov 13, 2020·World Journal of Stem Cells·Jagadish HosmaniHarish C Chandramoorthy
May 13, 2021·Clinical Oral Investigations·Yaghoup Feridouni KhamanehReinhard E Friedrich
Jul 3, 2021·International Journal of Molecular Sciences·Sarah Hani ShoushrahEdda Tobiasch

❮ Previous
Next ❯

Methods Mentioned

proteomic profiling
scanning electron microscopy

Software Mentioned

SPSS Statistics

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

ALS: Genetics

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder characterized by muscle weakness. Here is the latest research investigating genetic alterations in this genetically heterogeneous disorder.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

ALS: Genetics

Amyotrophic Lateral Sclerosis (ALS) is a progressive neurodegenerative disorder characterized by muscle weakness. ALS is a genetically heterogeneous disorder with several causative genes. Here are the latest discoveries pertaining to the genetics of this disease.

ALS: Pathogenic Mechanisms

Amyotrophic Lateral Sclerosis is a progressive neurodegenerative disorder characterized by muscle weakness. Here is the latest research investigating pathogenic mechanisms that underlie this genetically heterogeneous disorder.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.


Cardiomegaly, known as an enlarged heart, is a multifactorial disease with different pathophysiological mechanisms. Hypertension, pregnancy, exercise-induced and idiopathic causes are some mechanisms of cardiomegaly. Discover the latest research of cardiomegaly here.


TAR DNA-binding protein 43 (TDP-43) is a pathological protein identified in sporadic Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). Here are the latest discoveries pertaining to TDP-43 and these diseases.

Alzheimer's Disease: Tau & TDP-43

Alzheimer's disease is a neurodegenerative disease. This feed focuses on the underlying role of tau proteins and TAR DNA-binding protein 43, as well as other genetic factors, in Alzheimer's disease.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.

Related Papers

Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research
Manoj Kumar Hans, Shashit Shetty
Translational Research : the Journal of Laboratory and Clinical Medicine
F J Rodríguez-Lozano, J M Moraleda
© 2021 Meta ULC. All rights reserved