Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart

Science in China. Series C, Life Sciences
Jun WangAiQun Ma

Abstract

This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 27 proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury.

References

Dec 1, 1992·Cardiovascular Research·G D LopaschukT Itoi
Feb 1, 1987·The Journal of Clinical Investigation·J A WisneskiM Mayr
Jun 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C Thorpe, J J Kim
Apr 4, 2001·The Journal of Clinical Investigation·H C ChiuJ E Schaffer
Jul 2, 2002·The Journal of Thoracic and Cardiovascular Surgery·Yutaka SakakibaraMasashi Komeda
Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Lionel H Opie, Michael N Sack
Feb 20, 2003·Nature Biotechnology·Steven W TaylorSoumitra S Ghosh
Apr 1, 1954·The American Journal of Medicine·R J BINGM GILBERT
Jan 1, 1961·Scandinavian Journal of Clinical and Laboratory Investigation·A CARLSTENL WERKO
Aug 25, 2004·Circulation·Heinrich Taegtmeyer
Nov 16, 2004·Proteomics·Angelika GörgMichael J Dunn
Dec 17, 2004·Journal of Cellular and Molecular Medicine·Melissa R DentParamjit S Tappia
Mar 1, 2005·Journal of Molecular and Cellular Cardiology·Michael FountoulakisYassemi Capetanaki
Oct 18, 2005·Heart Failure Reviews·W B Kannel
Jun 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bjoern SchwerEric Verdin
Aug 2, 2006·Internal Medicine·Hiroyuki Tsutsui
Aug 25, 2006·American Journal of Physiology. Cell Physiology·D Thor JohnsonRobert S Balaban
Dec 20, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Andrew J MurrayKieran Clarke
Oct 16, 2008·Cardiovascular Research·Hiroyuki TsutsuiShouji Matsushima
Mar 4, 2009·Journal of Cardiac Failure·Alexandra Alberta SantosValdir A Moises

❮ Previous
Next ❯

Citations

Jul 8, 2011·European Journal of Applied Physiology·Wei-Zhong ZhuZhao-Nian Zhou
Dec 24, 2013·Cellular and Molecular Life Sciences : CMLS·Lawrence R GrayEric B Taylor
May 11, 2013·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Angela CintolesiRamon Gonzalez
Nov 23, 2011·Congestive Heart Failure·John M HollanderErinne R Dabkowski
Aug 16, 2011·Acta Pharmacologica Sinica·Zi-jian LiYou-yi Zhang
Oct 16, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Mark V PintiJohn M Hollander
Jul 25, 2021·International Journal of Environmental Research and Public Health·Weronika WasylukAgnieszka Zwolak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved