Proteomic Analysis of Paracoccidioides brasiliensis During Infection of Alveolar Macrophages Primed or Not by Interferon-Gamma

Frontiers in Microbiology
Edilânia Gomes Araújo ChavesCélia Maria de Almeida Soares

Abstract

Although members of the Paracoccidioides complex are not obligate intracellular pathogens, they present the ability to survive and multiply inside epithelial cells and phagocytes of mammals, which may favor the spread of the fungus in host tissues. Macrophages resident in the lung are the first line of defense against paracoccidioidomycosis (PCM), presenting mechanisms to control the pathogen dissemination through the granuloma formation or eliminating the fungus through phagocytosis. Phagocytosis triggers an oxidative burst, in which there is an increase in the production of toxic elements, derived from oxygen and nitrogen. The interior of the phagolysosome is a harsh environment to the internalized pathogens, since in addition to the oxygen and nitrogen reactive species, microorganisms face nutrient shortages and proteases activity. Through the NanoUPLC-MS E technology, we analyzed the proteomic response of Paracoccidioides brasiliensis during the infection of alveolar macrophages primed or not by interferon gamma (IFN-γ). At 6 hs post-infection, only (IFN-γ)-primed macrophages were able to kill the fungus. We observed the regulation of amino acids degradation, tricarboxylic acid cycle, respiratory chain, ATP synthesis, gly...Continue Reading

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Citations

Nov 22, 2019·Scientific Reports·Caroline Maria MarcosAna Marisa Fusco-Almeida
Aug 28, 2020·Frontiers in Microbiology·Guilherme PetitoCélia Maria de Almeida Soares
Mar 5, 2021·Microbiological Research·Lucas Nojosa OliveiraCélia Maria de Almeida Soares
Mar 9, 2021·Frontiers in Cellular and Infection Microbiology·Gustavo Giusiano
Nov 7, 2020·Microbes and Infection·Juliana S de CurcioCélia Maria de Almeida Soares
Aug 22, 2020·Fungal Genetics and Biology : FG & B·Lucas Nojosa OliveiraCélia Maria de Almeida Soares
Jul 8, 2021·Journal of Chemical Ecology·Deivid França FreitasClóvis de Paula Santos

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Methods Mentioned

BETA
glycosylation
fluorescence microscopy
Fluorescence

Software Mentioned

Excel
FBAT
Pedant
PLGS
AxioVision
ProteinLynx Global SERVER ( PLGS )
Expression E informatics
MassPivot
MultiExperiment Viewer
Spotfire

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