Proteomic Analysis of Pigeonpea (Cajanus cajan) Seeds Reveals the Accumulation of Numerous Stress-Related Proteins

Journal of Agricultural and Food Chemistry
Hari B KrishnanOmar Darwish

Abstract

Pigeonpea is one of the major sources of dietary protein for more than a billion people living in South Asia. This hardy legume is often grown in low-input and risk-prone marginal environments. Considerable research effort has been devoted by a global research consortium to develop genomic resources for the improvement of this legume crop. These efforts have resulted in the elucidation of the complete genome sequence of pigeonpea. Despite these developments, little is known about the seed proteome of this important crop. Here, we report the proteome of pigeonpea seed. To enable the isolation of maximum number of seed proteins, including those that are present in very low amounts, three different protein fractions were obtained by employing different extraction media. High-resolution two-dimensional (2-D) electrophoresis followed by MALDI-TOF-TOF-MS/MS analysis of these protein fractions resulted in the identification of 373 pigeonpea seed proteins. Consistent with the reported high degree of synteny between the pigeonpea and soybean genomes, a large number of pigeonpea seed proteins exhibited significant amino acid homology with soybean seed proteins. Our proteomic analysis identified a large number of stress-related proteins, ...Continue Reading

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Citations

Apr 17, 2018·Electrophoresis·Tamara MartinovićDjuro Josić
Dec 19, 2018·International Journal of Molecular Sciences·Tatiana MamontovaAndrej Frolov
Sep 3, 2018·Molecular Genetics and Genomics : MGG·Jimmy ObalaRajeev K Varshney
Jun 21, 2019·International Journal of Molecular Sciences·Jitendra KumarShiv Kumar
Apr 25, 2019·International Journal of Molecular Sciences·Hari B KrishnanSavithiry S Natarajan
Jan 3, 2021·Plant Foods for Human Nutrition·Eliana Isabel Fernández SosaMaría Victoria Avanza
Nov 8, 2018·Journal of Agricultural and Food Chemistry·Ahmed O WarsamePaola Tosi
Aug 5, 2017·Journal of Agricultural and Food Chemistry·Gabriela Barbosa RossiAna Carolina Maisonnave Arisi

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