Proteomic analysis of pollination-induced corolla senescence in petunia.

Journal of Experimental Botany
Shuangyi BaiMichelle L Jones

Abstract

Senescence represents the last phase of petal development during which macromolecules and organelles are degraded and nutrients are recycled to developing tissues. To understand better the post-transcriptional changes regulating petal senescence, a proteomic approach was used to profile protein changes during the senescence of Petuniaxhybrida 'Mitchell Diploid' corollas. Total soluble proteins were extracted from unpollinated petunia corollas at 0, 24, 48, and 72 h after flower opening and at 24, 48, and 72 h after pollination. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in non-senescing (unpollinated) and senescing (pollinated) corollas, and image analysis was used to determine which proteins were up- or down-regulated by the experimentally determined cut-off of 2.1-fold for P <0.05. One hundred and thirty-three differentially expressed protein spots were selected for sequencing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the identity of these proteins. Searching translated EST databases and the NCBI non-redundant protein database, it was possible to assign a putative identification to greater than 90% of these proteins. Many o...Continue Reading

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Citations

Sep 11, 2010·Sexual Plant Reproduction·Ján A MiernykMartin Hajduch
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Nov 19, 2014·BMC Plant Biology·Shaun R BroderickMichelle L Jones
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Methods Mentioned

BETA
electrophoresis
PCR
protein extraction
transgenic
ubiquitination
acetylation

Software Mentioned

SAS
Primer Quest
Cluster
tBLASTn
Primer Quest SciTools
Sequest
TreeView
PDQuest
Mascot
Image PDQuest

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