Proteomic analysis of polymeric salivary mucins: no evidence for MUC19 in human saliva

The Biochemical Journal
K RousseauD J Thornton

Abstract

MUC5B is the predominant polymeric mucin in human saliva [Thornton, Khan, Mehrotra, Howard, Veerman, Packer and Sheehan (1999) Glycobiology 9, 293-302], where it contributes to oral cavity hydration and protection. More recently, the gene for another putative polymeric mucin, MUC19, has been shown to be expressed in human salivary glands [Chen, Zhao, Kalaslavadi, Hamati, Nehrke, Le, Ann and Wu (2004) Am. J. Respir. Cell Mol. Biol. 30, 155-165]. However, to date, the MUC19 mucin has not been isolated from human saliva. Our aim was therefore to purify and characterize the MUC19 glycoprotein from human saliva. Saliva was solubilized in 4 M guanidinium chloride and the high-density mucins were purified by density-gradient centrifugation. The presence of MUC19 was investigated using tandem MS of tryptic peptides derived from this mucin preparation. Using this approach, we found multiple MUC5B-derived tryptic peptides, but were unable to detect any putative MUC19 peptides. These results suggest that MUC19 is not a major component in human saliva. In contrast, using the same experimental approach, we identified Muc19 and Muc5b glycoproteins in horse saliva. Moreover, we also identified Muc19 from pig, cow and rat saliva; the saliva of...Continue Reading

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Citations

Nov 16, 2010·American Journal of Respiratory Cell and Molecular Biology·Lingxiang ZhuYin Chen
Jun 10, 2010·Pediatric Research·Diego PreciadoMary C Rose
Oct 15, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Biswadip DasDavid J Culp
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Apr 4, 2020·Annual Review of Biochemistry·Gunnar C Hansson
Oct 8, 2017·Virology Journal·Anwar Suleman MallCorena de Beer
Jun 23, 2020·Journal of Proteomics·Elisabetta Chiaradia, Ingrid Miller

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