Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line.

Investigational New Drugs
Marta MongeRicardo Pérez-Tomás

Abstract

Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.

References

Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
Jan 31, 1998·The Journal of Biological Chemistry·N O KuM B Omary
Aug 15, 1998·The Journal of Biological Chemistry·T SatoS Ohkuma
Oct 29, 2000·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·T Stigbrand
Dec 5, 2000·Current Opinion in Biotechnology·J C Reed, K J Tomaselli
May 15, 2001·International Journal of Cancer. Journal International Du Cancer·A HarbottleF C Campbell
Jun 5, 2001·Life Sciences·B Montaner, R Pérez-Tomás
Dec 4, 2001·Journal of Inorganic Biochemistry·M S MelvinR A Manderville
Mar 7, 2002·Toxicology Letters·Beatriz Montaner, Ricardo Pérez-Tomás
Apr 26, 2002·Cell Death and Differentiation·R G Oshima
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Marek TchórzewskiNikodem Grankowski
Feb 7, 2003·Current Cancer Drug Targets·Beatriz Montaner, Ricardo Pérez-Tomás
Jul 23, 2003·Journal of Cellular Biochemistry·Qing-Yu He, Jen-Fu Chiu
Sep 5, 2003·Journal of the American Society for Mass Spectrometry·Sabrina Laugesen, Peter Roepstorff
Oct 14, 2003·Biochemical Pharmacology·Ricardo Pérez-TomásVanessa Soto-Cerrato
Jul 21, 2004·Mass Spectrometry Reviews·Bernd Thiede, Thomas Rudel
Mar 25, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Beatriz MontanerRicardo Pérez-Tomás

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Citations

Nov 29, 2007·Future Microbiology·Neil R WilliamsonGeorge P C Salmond
Nov 23, 2007·FEMS Microbiology Letters·Alice A Sertan-de GuzmanGisela P Concepcion
Feb 1, 2008·Proteomics. Clinical Applications·Franck BrouillardMario Ollero
Aug 1, 2009·Proteomics. Clinical Applications·Hermann Lage
Jun 28, 2007·Chemical Communications : Chem Comm·Rosa I Sáez DíazAlison Thompson
Feb 25, 2014·Applied Microbiology and Biotechnology·Nada StankovicJasmina Nikodinovic-Runic
Jun 22, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Hongrong XieLinsen Hu
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·San-Lang WangAnh Dzung Nguyen

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