Proteomic analysis of purified Newcastle disease virus particles.

Proteome Science
Xiangpeng RenYongchang Cao

Abstract

Newcastle disease virus (NDV) is an enveloped RNA virus, bearing severe economic losses to the poultry industry worldwide. Previous virion proteomic studies have shown that enveloped viruses carry multiple host cellular proteins both internally and externally during their life cycle. To address whether it also occurred during NDV infection, we performed a comprehensive proteomic analysis of highly purified NDV La Sota strain particles. In addition to five viral structural proteins, we detected thirty cellular proteins associated with purified NDV La Sota particles. The identified cellular proteins comprised several functional categories, including cytoskeleton proteins, annexins, molecular chaperones, chromatin modifying proteins, enzymes-binding proteins, calcium-binding proteins and signal transduction-associated proteins. Among these, three host proteins have not been previously reported in virions of other virus families, including two signal transduction-associated proteins (syntenin and Ras small GTPase) and one tumor-associated protein (tumor protein D52). The presence of five selected cellular proteins (i.e., β-actin, tubulin, annexin A2, heat shock protein Hsp90 and ezrin) associated with the purified NDV particles was...Continue Reading

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Citations

Aug 12, 2014·PloS One·Megan Moerdyk-SchauweckerValery Z Grdzelishvili
Jul 2, 2016·Biological Chemistry·Alexander KuehnlUrsula Rescher

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Methods Mentioned

BETA
electrophoresis
electron microscopy
GTPase

Software Mentioned

Image Master 2D Platinum
MASCOT
GPS Explorer

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