Proteomic analysis of rat pheochromocytoma PC12 cells

Proteomics
Wei YangJianguo Ji

Abstract

PC12 cell line is well documented and widely applied as many kinds of models in neurobiological and neurochemical studies. Yet a thorough proteomic analysis has not been performed so far. Here we report the construction of a large-scale 2-D protein database for PC12 cells. The proteins extracted from PC12 cells were separated by 2-DE and identified by MALDI-TOF/TOF MS. A total of 1080 protein spots, excised from three different 2-D gels, were identified with high confidence. These proteins represent 474 different gene products, mainly binding proteins and enzymes. Three hundred and seven identified protein spots were located in the low-molecular weight region below 20 kDa. This database today represents one of the largest 2-D databases for higher eukaryotic cell proteomes and for low-molecular weight proteins. In addition, fragment ion spectra obtained by TOF/TOF confirmed that calcylin in PC12 cells was N-acetylated. The database of PC12 proteome is expected to be a powerful tool for neuroscientists.

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Citations

Feb 6, 2009·Cellular and Molecular Neurobiology·Ramasamy SaminathanPonnampalam Gopalakrishnakone
May 22, 2013·Molecular BioSystems·Hongqin ZhuangZi-Chun Hua
Apr 9, 2011·Molecular & Cellular Proteomics : MCP·Jianxiang ChenZi-Chun Hua
Oct 6, 2009·Molecular & Cellular Proteomics : MCP·Wei YangMichael R Freeman
Nov 12, 2010·Journal of Virology·Zhiguo SunJun Gu
Feb 3, 2015·Journal of Cellular Physiology·Chun YaoZi-Chun Hua
Sep 12, 2015·Journal of Medical Virology·Xiaoqing ZhangJinfeng Zhao
Dec 18, 2007·Biochimica Et Biophysica Acta·Xiaolei XieJianguo Ji
Dec 8, 2011·Journal of Proteomics·Baikuntha P AryalMark P Jensen
Sep 1, 2007·Proteomics. Clinical Applications·Fernando VivancoMaria G Barderas
Nov 9, 2007·Journal of Proteome Research·Saleem A BokhariJin-Yuan Liu

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