Proteomic analysis of resistance mediated by Rcm 2.0 and Rcm 5.1, two loci controlling resistance to bacterial canker of tomato

Molecular Plant-microbe Interactions : MPMI
Gitta L CoakerDavid M Francis

Abstract

Two quantitative trait loci from Lycopersicon hirsutum, Rcm 2.0 and Rcm 5.1, control resistance to Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. Lines containing Rcm 2.0 and Rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to C. michiganensis subsp. michiganensis infection. A total of 47 proteins were subjected to tandem mass spectrometry. Database queries with resulting spectra identified tomato genes for 26 proteins. The remaining 21 proteins were either identified in other species or possessed no homology to known proteins. Spectra were interpreted to deduce peptide amino acid sequences that were then used to query publicly available data. This approach identified tomato genes or expressed sequence tags for 44 of the proteins analyzed. Three superoxide dismutase (SOD) enzymes were differentially regulated among genotypes, and patterns of hydrogen peroxide accumulation were genotype- and tissue-specific, indicating a role for oxidative stress in response to C. michiganensis subsp. michiganensis. Steady-state mRNA and protein levels for SOD, thioredoxin M-type...Continue Reading

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Citations

Mar 1, 2012·Journal of Proteome Research·Punit ShahGerardo Gutierrez-Sanchez
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Benoit LefebvreThomas Ott
Jan 30, 2010·Journal of Experimental Botany·Shuangyi BaiMichelle L Jones
May 21, 2013·Annual Review of Phytopathology·Dov PruskyRobert Fluhr
Dec 2, 2011·Journal of Biomedicine & Biotechnology·Tamara ZimaroJorgelina Ottado
Mar 29, 2011·Annual Review of Phytopathology·Rudolf Eichenlaub, Karl-Heinz Gartemann
May 29, 2012·Journal of Proteomics·Xianping FangSonglin Ruan
Dec 17, 2009·Phytochemistry·B F QuirinoR H Krüger
Jul 12, 2008·The FEBS Journal·Angela MehtaThales L Rocha
Feb 3, 2015·Proteomics·Xianping FangChengqi Yan
Dec 9, 2010·Molecular Plant-microbe Interactions : MPMI·Iris K Jarsch, Thomas Ott
Jul 25, 2019·Phytopathology·Qingyang LyuLaixin Luo
Aug 20, 2019·Cellular and Molecular Life Sciences : CMLS·Meng YuJinxing Lin
Oct 28, 2020·Plant Disease·F Christopher Peritore-GalveChristine D Smart

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