Proteomic analysis of synovial fluid: current and potential uses to improve clinical outcomes

Expert Review of Proteomics
Mandy Jayne PeffersJames Ross Anderson

Abstract

Synovial fluid (SF) is in close proximity to tissues which are primarily altered during articular disease and has significant potential to better understand the underlying disease pathogeneses of articular pathologies and biomarker discovery. Although development of mass spectrometry-based methods has allowed faster and higher sensitivity techniques, interrogation of the SF proteome has been hindered by its large protein concentration dynamic range, impeding quantification of lower abundant proteins. Areas covered: Recent advances have developed methodologies to reduce the large protein concentration dynamic range of SF and subsequently allow deeper exploration of the SF proteome. This review concentrates on methods to overcome biofluid complexity, mass spectrometry proteomics methodologies, extracellular vesicles proteomics and the application of advances within the field in clinical disease, including osteoarthritis, rheumatoid arthritis, spondyloarthritis and juvenile arthritis. A narrative review was conducted with articles searched using PubMed, 1991-2018. Expert opinion: The SF proteomics field faces various challenges, including the requirement for rigorous and standardised methods of sample collection/storage, the sensi...Continue Reading

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Citations

Aug 29, 2020·Bioelectricity·Shreedevi Kumar, Blanka Sharma
Dec 9, 2020·British Medical Bulletin·Emily J ClarkeMandy J Peffers
Apr 2, 2020·Journal of Proteome Research·James R AndersonMandy J Peffers
Jul 24, 2020·Journal of Proteome Research·James R AndersonMandy J Peffers
Mar 30, 2021·Mediators of Inflammation·Rajkamal KumavatSagarika Biswas

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