Proteomic analysis of tea plants (Camellia sinensis) with purple young shoots during leaf development

PloS One
Qiongqiong ZhouWeijiang Sun

Abstract

Tea products made from purple leaves are highly preferred by consumers due to the health benefits. This study developed a proteome reference map related to color changes during leaf growth in tea (Camellia sinensis) plant with purple young shoots using two-dimensional electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel and successfully identified by using MALDI-TOF/TOF-MS. The pronounced changes in the proteomic profile between tender purple leaves (TPL) and mature green leaves (MGL) included: 1) the lower activity of proteins associated with CO2 assimilation, energy metabolism and photo flux efficiency and higher content of anthocyanins in TPL than those in MGL may protect tender leaves against photo-damage; 2) the higher abundance of chalcone synthase (CHS), chalcone isomerase (CHI) and flavonol synthase (FLS) likely contributes to the synthesis of anthocyanins, catechins and flavonols in TPL tissues; 3) higher abundance of stress response proteins, such as glutathione S-transferases (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx), could enhance the tolerance of TPL tissues to adverse condition in; and 4) the increased abundance of proteins related to protein synthe...Continue Reading

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Citations

Nov 23, 2019·Journal of the Science of Food and Agriculture·Qunfeng ZhangJianyun Ruan
Dec 20, 2019·Planta·Manglesh KumariRajiv Kumar
Mar 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dylan O'Neill RothenbergLingyun Zhang

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Methods Mentioned

BETA
electrophoresis
PCA
transgenic
PCR

Software Mentioned

TOF
Camo
MASCOT
GPS Explorer
Unscrambler
TOF [UNK] SCIEX
PDQuest
The Unscrambler

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