Proteomic Analysis of the Acid-Insoluble Fraction of Whole Saliva from Patients Affected by Different Forms of Non-histaminergic Angioedema.

Journal of Clinical Immunology
Davide FirinuMaria T Sanna

Abstract

We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema. Graphical Abstract Graphical Abstract.

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Citations

Aug 6, 2021·Journal of Separation Science·Mozhgan BoroumandMassimo Castagnola

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Methods Mentioned

BETA
glycosylation
electrophoresis
bronchoalveolar lavage

Software Mentioned

SameSpots
SEQUEST
TotalLab
Proteome Discoverer
SameSpots Multivariate Stats
GraphPad Prism

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