Abstract
The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of plastic adherent, MSC subpopulation, of bone marrow cells (BMC). Effects of serial culture on MSC phenotype were investigated using non-gel based quantitative proteomic methodology for static monolayer cultures of rat BMC. In total, 382 proteins were relatively quantified (≥ 2 peptides). Nine proteins were up-regulated and seven down-regulated at passage 4 relative to passage 2 (p ≤ 0.05). We propose that serial culture impacts on MSC expansion (observed decline in colony forming potential and colony size) is through a combination of osteogenic differentiation and ageing/senescence and propose six novel protein biomarkers as candidates for quality control purposes in bioprocessing.
References
Feb 1, 1997·Journal of Cellular Biochemistry·S P BruderS E Haynesworth
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 14, 2005·Molecular & Cellular Proteomics : MCP·Yi ZhangForest M White
Jan 20, 2006·Proteomics·Hyun-Kyung LeeChan-Wha Kim
Jul 18, 2006·Aging Cell·Alexandra Stolzing, Andrew Scutt
Aug 23, 2006·Cytotherapy·M DominiciEm Horwitz
Sep 2, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hyun Jin SunJin Woo Lee
May 29, 2007·Molecular and Cellular Biochemistry·Ai-Xia ZhangAndy Peng Xiang
Jun 23, 2007·BMC Bioinformatics·Andreas M BoehmMichael Falk
Jul 27, 2007·Proteomics·Hye Won ParkChan-Wha Kim
Feb 1, 2008·Journal of Proteome Research·Adam GlenIshtiaq Rehman
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Ji-Soo KimChan-Wha Kim
Nov 27, 2008·Nucleic Acids Research·Da Wei HuangRichard A Lempicki
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 20, 2009·Journal of Cell Science·Dmitry Yudin, Mike Fainzilber
Mar 28, 2009·Journal of Proteome Research·Betül Celebi, Y Murat Elçin
Sep 2, 2010·Proteomics·Trong Khoa PhamGraham P Stafford