Proteomic analysis of the impact of static culturing on the expansion of rat bone marrow mesenchymal stem cells

Biotechnology Letters
Stefan PeterCatherine A Biggs

Abstract

The clinical potential of mesenchymal stem cells (MSC) in tissue engineering and regenerative medicine is due to their self-renewal, proliferation and multi-lineage differentiation potential. Clinical use requires large cell numbers; which can, theoretically, be generated by ex vivo expansion of plastic adherent, MSC subpopulation, of bone marrow cells (BMC). Effects of serial culture on MSC phenotype were investigated using non-gel based quantitative proteomic methodology for static monolayer cultures of rat BMC. In total, 382 proteins were relatively quantified (≥ 2 peptides). Nine proteins were up-regulated and seven down-regulated at passage 4 relative to passage 2 (p ≤ 0.05). We propose that serial culture impacts on MSC expansion (observed decline in colony forming potential and colony size) is through a combination of osteogenic differentiation and ageing/senescence and propose six novel protein biomarkers as candidates for quality control purposes in bioprocessing.

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Citations

Nov 20, 2012·Biotechnology Letters·Stefan PeterCatherine A Biggs
Jun 29, 2013·Drug Discovery Today·Daniela CigogniniDimitrios Zeugolis

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