Proteomic analysis of the photosystem I light-harvesting antenna in tomato (Lycopersicon esculentum)

Biochemistry
Stefanie StorfVolkmar H R Schmid

Abstract

Until now, more genes of the light-harvesting antenna of higher-plant photosystem I (PSI) than proteins have been described. To improve our understanding of the composition of light-harvesting complex I (LHCI) of tomato (Lycopersicon esculentum), we combined one- and two-dimensional (1-D and 2-D, respectively) gel electrophoresis with immunoblotting and tandem mass spectrometry (MS/MS). Separation of PSI with high-resolution 1-D gels allowed separation of five bands attributed to proteins of LHCI. Immunoblotting with monospecific antibodies and MS/MS analysis enabled the correct assignment of the four prominent bands to light-harvesting proteins Lhca1-4. The fifth band was recognized by only the Lhca1 antibody. Immunodetection as well as mass spectrometric analysis revealed that these protein bands contain not only the eponymous protein but also other Lhca proteins, indicating a heterogeneous protein composition of Lhca bands. Additionally, highly sensitive MS/MS allowed detection of a second Lhca4 isoform and of Lhca5. These proteins had not been described before on the protein level in higher plants. Two-dimensional gel electrophoresis revealed an even more diverse composition of individual Lhca proteins than was apparent fro...Continue Reading

Citations

Jul 3, 2010·Photosynthesis Research·Jonathan A D Neilson, Dion G Durnford
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Nathan Nelson, Adam Ben-Shem
Nov 20, 2009·The Journal of Biological Chemistry·Alexey AmuntsNathan Nelson
Jan 14, 2009·The Journal of Biological Chemistry·Emilie WientjesRoberta Croce
Nov 17, 2006·FEBS Letters·Robert LucinskiFrank Klimmek
Jul 13, 2012·Journal of Plant Physiology·Joanna KargulTomasz Krupnik
Aug 19, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nathan Nelson, Adam Ben-Shem
Apr 28, 2007·Proteomics·Lello ZollaAnna Maria Timperio
Apr 26, 2005·Biophysical Chemistry·Zhenle YangTingyun Kuang
Oct 6, 2010·Biochimica Et Biophysica Acta·Andreas Busch, Michael Hippler
Nov 26, 2004·The Journal of Biological Chemistry·Stefanie StorfVolkmar H R Schmid
Jun 7, 2006·The Journal of Biological Chemistry·Antje WehnerPeter Jahns
Sep 14, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Xue LiangYi Cao

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