Proteomic analysis of the presynaptic active zone.

Experimental Brain Research
Walter Volknandt, Michael Karas

Abstract

Synaptic vesicles are key organelles in chemical signaling, allowing neurons to communicate with each other and with neighboring cells. Vesicle integral or membrane-associated proteins mediate the various tasks the organelle fulfills during its life cycle. These include organelle transport, interaction with the nerve terminal cytoskeleton, uptake and storage of low molecular weight constituents, and the regulated interaction with the presynaptic plasma membrane, the active zone, during exo- and endocytosis. Converging work from several laboratories within the last 30 years resulted in the molecular and functional characterization of the protein inventory of the synaptic vesicle compartment. Nowadays advances in membrane protein separation and mass spectrometry have dramatically promoted this field resulting in a detailed description of the synaptic vesicle proteome and making synaptic vesicles the best characterized organelles. Recently, the proteome of the active zone was identified using the docked synaptic vesicles as target for immunoisolation. Combining gel-based protein separation techniques, mass spectrometry, and immunodetection, a considerable variety of proteins has been detected in the active zone. This includes syna...Continue Reading

References

Dec 15, 1987·Biochemistry·T FunakoshiK Fujikawa
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G C OwensB A Cunningham
Nov 1, 1984·The Journal of Cell Biology·B RanschtC Thomas
Nov 1, 1994·Journal of Neurochemistry·M InuiK Sobue
Jun 1, 1994·Trends in Biochemical Sciences·R D Burgoyne, M J Clague
Sep 13, 1996·Biochemical and Biophysical Research Communications·T KameyamaH Fujisawa
Sep 13, 1996·Biochemical and Biophysical Research Communications·T KameyamaH Fujisawa
Jan 10, 1997·The Journal of Biological Chemistry·K LangnaeseE D Gundelfinger
Mar 15, 1997·Genomics·H OhnishiS Sano
Jul 14, 1997·FEBS Letters·S SanoM Kubota
Aug 1, 1997·Neuron·M MenegozJ A Girault
Mar 14, 1998·The Journal of Cell Biology·C J JengE S Schweitzer
Oct 16, 1999·Cell·T TakahashiS M Strittmatter
Feb 17, 2000·Neurochemistry International·E J CoulsonC L Masters
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·K H SmallaE D Gundelfinger

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Citations

Feb 22, 2012·Experimental Brain Research·Ulrike C MüllerThomas Deller
Jun 11, 2014·Cellular and Molecular Life Sciences : CMLS·John Jia En Chua
Feb 27, 2014·Biochemistry·Katherine M BetkeHeidi E Hamm
Jul 3, 2013·Journal of Neurochemistry·Melanie LaßekWalter Volknandt
Nov 25, 2014·Proteomics. Clinical Applications·Joaquín Fernandez-IrigoyenEnrique Santamaria
Dec 3, 2014·Journal of Neurochemistry·Lei LiuMasaki Nishimura
May 1, 2013·Cell Adhesion & Migration·Jessica C NelsonDaniel A Colón-Ramos
Jul 21, 2014·Cell and Tissue Research·Melanie LaßekWalter Volknandt
Jan 18, 2018·Biological Chemistry·Benedikt BeckertWalter Volknandt
Apr 24, 2014·Proteomes·Melanie LaßekWalter Volknandt
May 20, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Guzalia F ZakyrjanovaAlexey M Petrov
Oct 9, 2021·Neurochemical Research·Joao Paulo Cavalcanti-de-AlbuquerqueAntonio Galina

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