Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model

Proteomics
Fuzheng GuoDacheng He

Abstract

The 70% (or 2/3) partial hepatectomy (PHx) rat liver model provides an effective medium for study of the transition and regulation of hepatocytes from quiescent to proliferating phase. Although the gene expression pattern has come under intense scrutiny, a differential proteomic study could help to reveal the mechanism of how the process is initiated and regulated. The proteomic changes were analyzed in two groups, 7 h after 70% PHx test group and sham-operation control group, by two-dimensional gel electrophoresis with 907 +/- 33 and 910 +/- 64 spots on gels, respectively. Twelve down-regulated spots and twenty-six up-regulated spots were recognized using ImageMaster software and were identified by matrix-assisted laser desorption/ionization-mass spectrometry-quadrupole time of flight mass spectrometry and/or tandem mass spectrometry reconfirmation. Some of the differential proteins were associated with stress defense, lipid metabolism, and macromolecular biosynthesis while the others were shown to be involved in regulating transcript factors associated with liver regeneration. A "proteomic model" for liver regeneration was suggested based on our data and related scientific literature to interpret the differential proteome pat...Continue Reading

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Citations

Nov 18, 2010·Development Genes and Evolution·Cunshuan XuFuchun Zhang
Jan 26, 2011·Cellular and Molecular Neurobiology·Cunshuan XuAiling Lu
May 14, 2011·Journal of Proteome Research·Valeria SeverinoAngela Chambery
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Dec 23, 2008·Journal of Proteome Research·Hui-Chu HsiehSung-Fang Chen

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