Proteomic analysis of the venom from the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Archives of Insect Biochemistry and Physiology
Jia-ying ZhuGongyin Ye

Abstract

Parasitoid venom is a complex mixture of active substances with diversified biological functions. Because of its range of activities, venom is an important resource with respect to potential application in agriculture and medicine. Only a limited number of peptides, proteins, and enzymes have been identified and characterized from parasitoid venom. Here we describe a proteomic analysis of the venom from the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae). Venom resolved by two-dimensional electrophoresis yielded 56 protein spots with major proteins in the pI range 4-7 and molecular mass range of 25-66.2 kDa. The amino acid sequences of the proteins were identified by mass spectrometry. Several venom proteins such as calreticulin, venom acid phosphatase, serine protease, arginine kinase, serine protease homolog, aminotransferase-like venom protein, and heat shock protein 70, were identified in silico based on their amino acid sequences. The full-length cDNAs of calreticulin and arginine kinase were cloned. Calreticulin showed 62% identity with calreticulin in the venom of Cotesia rubecula. Arginine kinase showed a high level of sequence identity (92%) with its counterpart in the venom of Cyphononyx dorsalis. R...Continue Reading

References

Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·J LelukD Jones
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·B J VisserT Piek
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Aug 7, 1998·The International Journal of Biochemistry & Cell Biology·M G Coppolino, S Dedhar
Dec 5, 2000·Insect Biochemistry and Molecular Biology·N ParkinsonJ P Edwards
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M BinderM Duchêne
Mar 13, 2002·Developmental and Comparative Immunology·J Y ChoiB L Lee
May 23, 2003·Archives of Insect Biochemistry and Physiology·Sassan AsgariOtto Schmidt
May 29, 2003·Journal of Insect Physiology·James B Whitfield, Sassan Asgari
Jun 14, 2003·Journal of Insect Physiology·S Asgari, O Schmidt
Sep 25, 2003·Insect Biochemistry and Molecular Biology·Sassan AsgariOtto Schmidt
Dec 4, 2003·Annual Review of Entomology·Jacques Brodeur, Guy Boivin
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Oct 9, 2004·Science·Eric EspagneJean Michel Drezen
Jan 1, 2005·Veterinary Immunology and Immunopathology·John D Lippolis, Timothy A Reinhardt
Jun 7, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M P DaniE H Richards
Oct 6, 2005·Insect Biochemistry and Molecular Biology·S J M Moreau, S Guillot
Dec 8, 2005·Annual Review of Entomology·Francesco Pennacchio, Michael R Strand
Dec 21, 2005·Developmental and Comparative Immunology·Guangmei ZhangSassan Asgari
Feb 17, 2006·Archives of Insect Biochemistry and Physiology·Sassan Asgari
May 19, 2006·Pathophysiology of Haemostasis and Thrombosis·R Manjunatha Kini
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Tsuyoshi YamamotoDaisuke Uemura
Apr 26, 2007·Insect Biochemistry and Molecular Biology·Patrizia FalabellaFrancesco Pennacchio
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 15, 2007·International Archives of Allergy and Immunology·Karina D García-OrozcoRogerio R Sotelo-Mundo
Aug 11, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qing-Yun WuXiao-Yun Wang
Nov 16, 2007·Nucleic Acids Research·Nicolas HuloChristian J A Sigrist
Jan 2, 2008·Micron : the International Research and Review Journal for Microscopy·Jia-Ying ZhuCui Hu
May 2, 2008·Toxicon : Official Journal of the International Society on Toxinology·Jia-ying ZhuCui Hu
May 15, 2008·Insect Molecular Biology·A M CrawfordA McCulloch
Jan 13, 2009·The Biochemical Journal·Marek MichalakMichal Opas

❮ Previous
Next ❯

Citations

May 30, 2013·PloS One·Jeremy GoecksTodd A Schlenke
Jul 2, 2015·Toxins·Sébastien J M Moreau, Sassan Asgari
Dec 27, 2015·Journal of Insect Physiology·Antoine GuiguetDavid Giron
Oct 30, 2012·Journal of Insect Physiology·Dominique ColinetMarylène Poirié
Oct 18, 2012·Journal of Insect Physiology·Sébastien J M Moreau
Jan 5, 2014·Molecular Ecology·Gaelen R Burke, Michael R Strand
Jul 22, 2014·Archives of Insect Biochemistry and Physiology·Jia-Ying ZhuBin Yang
Apr 26, 2016·International Journal of Molecular Sciences·Wallace Felipe Blohem PessoaCarla Cristina Romano
Sep 17, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Atif ManzoorAmer Jamil
Apr 22, 2015·Scientific Reports·Zhirui LiuYonghua Ji
Jun 28, 2017·Toxicon : Official Journal of the International Society on Toxinology·Nai-Yong LiuJia-Ying Zhu
Aug 26, 2017·Evolution; International Journal of Organic Evolution·Alice B DennisChristoph Vorburger
Aug 10, 2019·Frontiers in Physiology·Sébastien CambierJean-Michel Drezen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.