Aug 8, 2018

Proteomic Analysis of Ubiquitinated Proteins in Rice (Oryza sativa ) After Treatment With Pathogen-Associated Molecular Pattern (PAMP) Elicitors

Frontiers in Plant Science
Xiao-Lin ChenWende Liu

Abstract

Reversible protein ubiquitination plays essential roles in regulating cellular processes. Although many reports have described the functions of ubiquitination in plant defense responses, few have focused on global changes in the ubiquitome. To better understand the regulatory roles of ubiquitination in rice pattern-triggered immunity (PTI), we investigated the ubiquitome of rice seedlings after treatment with two pathogen-associated molecular patterns, the fungal-derived chitin or the bacterial-derived flg22, using label-free quantitative proteomics. In chitin-treated samples, 144 and 167 lysine-ubiquitination sites in 121 and 162 proteins showed increased and decreased ubiquitination, respectively. In flg22-treated samples, 151 and 179 lysine-ubiquitination sites in 118 and 166 proteins showed increased and decreased ubiquitination, respectively. Bioinformatic analyses indicated diverse regulatory roles of these proteins. The ubiquitination levels of many proteins involved in the ubiquitination system, protein transportation, ligand recognition, membrane trafficking, and redox reactions were significantly changed in response to the elicitor treatments. Notably, the ubiquitination levels of many enzymes in the phenylpropanoid m...Continue Reading

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Mentioned in this Paper

YME1L1 gene
Biochemical Pathway
Patterns
Rice (Dietary)
Regulation of Cellular Process
Enzymes, antithrombotic
Pathogenic Organism
Ubiquitin
Oryza sativa
Ubiquitinated Proteins

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