Proteomic analysis of wild-type and mutant huntingtin-associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis.

The Journal of Biological Chemistry
Brady P CulverNaoko Tanese

Abstract

Huntington disease is a neurodegenerative disorder caused by a CAG repeat amplification in the gene huntingtin (HTT) that is reflected by a polyglutamine expansion in the Htt protein. Nearly 20 years of research have uncovered roles for Htt in a wide range of cellular processes, and many of these discoveries stemmed from the identification of Htt-interacting proteins. However, no study has employed an impartial and comprehensive strategy to identify proteins that differentially associate with full-length wild-type and mutant Htt in brain tissue, the most relevant sample source to the disease condition. We analyzed Htt affinity-purified complexes from wild-type and HTT mutant juvenile mouse brain from two different biochemical fractions by tandem mass spectrometry. We compared variations in protein spectral counts relative to Htt to identify those proteins that are the most significantly contrasted between wild-type and mutant Htt purifications. Previously unreported Htt interactions with Myo5a, Prkra (PACT), Gnb2l1 (RACK1), Rps6, and Syt2 were confirmed by Western blot analysis. Gene Ontology analysis of these and other Htt-associated proteins revealed a statistically significant enrichment for proteins involved in translation ...Continue Reading

References

Jun 8, 1965·Biochimica Et Biophysica Acta·N SilmanH Engleberg
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·E BrouilletM F Beal
Dec 1, 1993·Human Molecular Genetics·A T HoogeveenH Galjaard
Mar 1, 1996·Annals of Neurology·M GuA H Schapira
Oct 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C de HaroJ Santoyo
Mar 1, 1997·Human Molecular Genetics·E E WankerH Lehrach
Aug 4, 1998·The EMBO Journal·R C Patel, G C Sen
Aug 13, 1998·Human Molecular Genetics·P W FaberM E MacDonald
Nov 25, 2000·Human Molecular Genetics·F TrettelM E MacDonald
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·S HolbertC Néri
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peter W FaberAnne C Hart
Mar 21, 2003·Journal of Proteome Research·Rovshan G SadygovJohn R Yates
Aug 20, 2003·The Journal of Comparative Neurology·Liliana B MenalledMarie-Françoise Chesselet
Sep 4, 2003·Genome Research·Paul D ThomasApurva Narechania
Jul 21, 2004·Bioinformatics·Marshall BernJohn R Yates
Mar 1, 2006·The Journal of Cell Biology·Ai YamamotoJames E Rothman
Jul 5, 2006·Nature Neuroscience·Hiroko YanoMoses V Chao
Dec 5, 2006·Current Biology : CB·Atsushi YoshimuraToru Takumi
May 16, 2007·PLoS Genetics·Linda S KaltenbachRobert E Hughes
Oct 24, 2007·Sub-cellular Biochemistry·Fengju Bai, Frank A Witzmann
Mar 18, 2008·Nature Methods·Sung Kyu ParkJohn R Yates
Apr 23, 2008·Current Protocols in Neuroscience·Penelope J HallettAnthone W Dunah
May 10, 2008·The Biochemical Journal·Sara ImarisioDavid C Rubinsztein
Jul 23, 2008·Molecular & Cellular Proteomics : MCP·Hyungwon ChoiAlexey I Nesvizhskii
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey N SavasNaoko Tanese

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Citations

May 24, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Barbara CalaminiLinda S Kaltenbach
Dec 22, 2012·Analytical Chemistry·Sergei V SavelievMarjeta Urh
May 3, 2013·Genome Medicine·Uros Kuzmanov, Andrew Emili
Feb 8, 2014·PloS One·Erin E WatkinChristopher A Ross
Dec 11, 2013·Molecular Neurobiology·Ramavati PalSimon Geir Møller
Dec 3, 2014·Molecular and Cellular Neurosciences·Jia YaoSandra Bajjalieh
Feb 5, 2013·Journal of Proteomics·Narkhyun BaeGert Lubec
Jan 7, 2016·Frontiers in Molecular Neuroscience·Anne BieverEmma Puighermanal
Mar 5, 2016·Neuron·Frédéric Saudou, Sandrine Humbert
Oct 8, 2014·Proteomics. Clinical Applications·Eva KotrcovaHana Kovarova
Feb 19, 2016·Journal of Huntington's Disease·Brady P CulverNaoko Tanese
Apr 14, 2016·European Journal of Cell Biology·Kamalika Roy ChoudhuryNitai P Bhattacharyya
Dec 3, 2013·Journal of Molecular Biology·Karunakar KarRonald Wetzel
Mar 19, 2014·Drug Discovery Today·Constanza J Cortes, Albert R La Spada
Feb 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zahra DargaeiMelanie A Woodin
Aug 8, 2018·International Journal of Molecular Sciences·Stanislav A BondarevGalina A Zhouravleva
Jan 11, 2014·The Journal of Biological Chemistry·Cendrine TouretteRobert E Hughes
May 30, 2019·Molecular & Cellular Proteomics : MCP·Karen A SapEric A Reits
Jul 13, 2019·Neurotoxicity Research·Sachchida Nand RaiSurya Pratap Singh
Mar 25, 2017·Journal of Huntington's Disease·Jeh-Ping Liu, Scott O Zeitlin
Jul 14, 2017·Scientific Reports·Ravi Kiran Reddy KalathurMatthias E Futschik
Jun 28, 2018·Cellular and Molecular Life Sciences : CMLS·Chang Geon ChungSung Bae Lee
Dec 31, 2018·Journal of Huntington's Disease·Adelaide TousleyKimberly B Kegel-Gleason
Apr 5, 2020·International Journal of Molecular Sciences·Franz MarxreiterZacharias Kohl
Mar 25, 2017·Frontiers in Molecular Neuroscience·Marta Martinez-Vicente
Dec 7, 2018·Open Biology·Yi-Ting HsuYijuang Chern
Jul 28, 2020·International Journal of Molecular Sciences·Ching-On Wong
Aug 31, 2017·Neural Regeneration Research·Leah Kershner, Kristy Welshhans
Dec 5, 2019·Cellular and Molecular Life Sciences : CMLS·Hiranmay JoagAmitabha Majumdar
Jun 12, 2018·Journal of Huntington's Disease·Morgane Sonia Thion, Sandrine Humbert

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