Proteomic analysis reveals a protective role of specific macrophage subsets in liver repair

Scientific Reports
Wenting YangLi Tang

Abstract

Macrophages are a heterogeneous population of immune cells that play central roles in a broad range of biological processes, including the resolution of inflammation. Although diverse macrophage subpopulations have been identified, the characterization and functional specialization of certain macrophage subsets in inflamed tissues remain unclear. Here we uncovered a key role of specific macrophage subsets in tissue repair using proteomics, bioinformatics and functional analysis. We isolated two hepatic monocyte-derived macrophage subpopulations: Ly6ChiCX3CR1lo macrophages and Ly6CloCX3CR1hi macrophages during distinct phases of acute liver injury and employed label-free proteomics approach to profile the proteome of these cells. We found that the endocytosis- and apoptotic cell clearance-related proteins were specifically enriched in Ly6CloCX3CR1hi macrophages at the resolution phase. Intriguingly, 12/15-lipoxygenase (Alox15), the most strongly up-regulated protein in Ly6CloCX3CR1hi macrophages, was identified as a specific marker for these macrophages. In co-culture systems, Ly6CloCX3CR1hi macrophages specifically induced hepatocyte proliferation. Furthermore, selective depletion of this population in CD11b-diphtheria toxin re...Continue Reading

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Citations

Jul 25, 2019·Frontiers in Pharmacology·Ryan G Snodgrass, Bernhard Brüne
Apr 18, 2021·Science Advances·Xiaokun WangJennifer H Elisseeff
Dec 7, 2021·American Journal of Physiology. Renal Physiology·John R MontfordSeth B Furgeson

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Methods Mentioned

BETA
proteomic profiling
flow cytometry
PCR
Flow
FACS
reversed phase chromatography

Software Mentioned

Exactive
Maxquant
Zeiss ZEN
Perseus
DAVID
GraphPad Prism
ImageJ
FlowJo

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