Proteomic analysis reveals changes in carbohydrate and protein metabolism associated with broiler breast myopathy

Poultry Science
V A KuttappanB M Hargis

Abstract

White Striping (WS) and Woody Breast (WB) are 2 conditions that adversely affect consumer acceptance as well as quality of poultry meat and meat products. Both WS and WB are characterized with degenerative myopathic changes. Previous studies showed that WS and WB in broiler fillets could result in higher ultimate pH, increased drip loss, and decreased marinade uptake. The main objective of the present study was to compare the proteomic profiles of muscle tissue (n = 5 per group) with either NORM (no or few minor myopathic lesions) or SEV (with severe myopathic changes). Proteins were extracted from these samples and analyzed using a hybrid LTQ-OrbitrapXL mass spectrometer (LC-MS/MS). Over 800 proteins were identified in the muscle samples, among which 141 demonstrated differential (P < 0.05) expression between NORM and SEV. The set of differentially (P < 0.05) expressed proteins was uploaded to Ingenuity Pathway Analysis® (IPA) software to determine the associated biological networks and pathways. The IPA analysis showed that eukaryotic initiation factor-2 (eIF-2) signaling, mechanistic target of rapamycin (mTOR) signaling, as well as regulation of eIF4 and p70S6K signaling were the major canonical pathways up-regulated (P < 0....Continue Reading

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Citations

Nov 28, 2018·Korean Journal for Food Science of Animal Resources·Xi Huang, Dong Uk Ahn
Aug 28, 2018·Asian-Australasian Journal of Animal Sciences·Yuwares MalilaWonnop Visessanguan
Sep 15, 2018·Journal of Animal Science and Biotechnology·Marco ZampigaFederico Sirri
Jul 17, 2020·Frontiers in Physiology·Christophe PraudCecile Berri
Jun 14, 2019·Frontiers in Physiology·Barbara de Almeida MallmannGuillermo Tellez-Isaias
Sep 26, 2020·Frontiers in Physiology·Richard A BaileySantiago Avendano
Dec 6, 2020·Animals : an Open Access Journal From MDPI·Krittaporn V ThanatsangWonnop Visessanguan
Feb 22, 2021·Poultry Science·Xue ZhangM Wes Schilling
May 6, 2021·Poultry Science·Xue ZhangM Wes Schilling
Aug 13, 2021·Frontiers in Physiology·Caroline Michele Marinho MarcianoMônica Corrêa Ledur
Aug 10, 2021·Frontiers in Physiology·Byungwhi KongCasey Owens
Sep 3, 2021·Journal of Food Science and Technology·Miriane Moreira Fernandes SantosFábio Anderson Pereira da Silva
Oct 7, 2021·Tropical Animal Health and Production·Muhammad Adnan AslamӦzay Güleş
Nov 2, 2021·Frontiers in Physiology·Denise R RodriguesLisa R Bielke

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