Proteomic and gene expression analysis of zebrafish brain undergoing continuous light/dark stress

Journal of Sleep Research
Sruthi PurushothamanMohammed M Idris

Abstract

Several organisms irrespective of their complexity in structure and function have an inbuilt circadian rhythm. Zebrafish could be used as an alternate model animal in sleep research as it exhibits similar sleep-wake dynamics as mammals and Drosophila. In this study, we have analysed the adult zebrafish brain for its differential proteome and gene expression during perturbed light/dark cycle. A total of 53 and 25 proteins including sncb, peroxiredoxins and TCR alpha were identified based on two-dimensional gel electrophoresis Fourier transform mass spectrometer/ion trap tandem mass spectrometer and differential in-gel electrophoresis MALDI TOF MS/MS analysis, respectively, with at least 1.5-fold changes between the control and experimental brains. Real time-polymerase chain reaction revealed that many circadian pathway-associated genes, such as per1b, bmal1b, cry1b, bmal2 and nr1d2, were differentially regulated during continuous light/dark exposures. It is hypothesized that the differential regulation of these genes might lead to the discovery of potential diagnostic markers for gaining insight into the light/dark-associated stress in humans.

References

Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jul 1, 1981·Endocrinology·M KanekoM F Dallman
Apr 10, 1999·Nature Neuroscience·D WhitmoreP Sassone-Corsi
Mar 9, 2000·Neuron·J C HendricksA I Pack
Mar 10, 2000·Science·P J ShawG Tononi
May 31, 2001·Brain Research·I V ZhdanovaN P Danilova
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·M P PandoP Sassone-Corsi
May 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Matthew P Pando, Paolo Sassone-Corsi
Jul 12, 2002·Cell and Tissue Research·Gregory M Cahill
Jan 1, 1960·Cold Spring Harbor Symposia on Quantitative Biology·J ASCHOFF
Mar 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jan KaslinPertti Panula
Sep 15, 2004·Neurology·H OhtakeA R La Spada
Oct 14, 2005·EMBO Reports·Martha MerrowTill Roenneberg
Jul 27, 2006·The Journal of Biological Chemistry·Juliette H FaracoEmmanuel Mignot
Dec 21, 2006·Epidemiology·Michael ConlonNancy Kreiger
Dec 22, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David A ProberAlexander F Schier
Mar 14, 2007·Scandinavian Journal of Work, Environment & Health·Yuko MorikawaKoji Nogawa
Oct 19, 2007·PLoS Biology·Tohei YokogawaEmmanuel Mignot
Mar 10, 2009·Current Neurology and Neuroscience Reports·Laura K BargerChristopher P Landrigan
May 5, 2009·Nature Genetics·Joachim HallmayerEmmanuel Mignot
May 14, 2009·Mass Spectrometry Reviews·Morten MøllerHenrik Vorum
Oct 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jason R GerstnerGregg W Roman
Oct 27, 2009·Endocrine Reviews·Oren Froy
Nov 21, 2009·Trends in Cell Biology·Akhilesh B Reddy, John S O'Neill
Jan 29, 2011·Nature·John S O'Neill, Akhilesh B Reddy
Mar 11, 2011·PloS One·Benjamin D WegerThomas Dickmeis
Jan 27, 2012·Molecular & Cellular Proteomics : MCP·Sandeep SaxenaMohammed M Idris
Sep 3, 2013·Behavioural Brain Research·Benjamín SigurgeirssonKarl Karlsson
Dec 7, 2013·Frontiers in Neural Circuits·Amanda SorribesKarl Æ Karlsson

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Citations

Sep 16, 2015·Aquatic Toxicology·Allan V KalueffUNKNOWN International Zebrafish Neuroscience Research Consortium ZNRC
Feb 22, 2017·British Journal of Pharmacology·Kanza M KhanDavid J Echevarria
Oct 13, 2017·Zebrafish·Sreeja SarasammaChung-Der Hsiao
Jan 16, 2018·Nuclear Receptor Research·Shari BodofskyBruce Wightman

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