Proteomic and histopathological characterisation of sicca subjects and primary Sjögren's syndrome patients reveals promising tear, saliva and extracellular vesicle disease biomarkers

Arthritis Research & Therapy
Lara A AqrawiJanicke Liaaen Jensen

Abstract

Mononuclear cell infiltration of exocrine glands, production of Ro/SSA and La/SSB autoantibodies, along with oral and ocular dryness, are characteristic features of primary Sjögren's syndrome (pSS). Non-SS sicca subjects, an underexplored group in relation to pSS, display similar sicca symptoms, with possible mild signs of inflammation in their salivary glands, yet with no serological detection of autoantibody production. In this study, we investigated inflammatory manifestations in the salivary gland tissue, tear fluid and saliva of non-SS subjects, as compared to pSS patients and healthy individuals. Fifteen non-SS, 10 pSS and 10 healthy subjects were included in the analyses. Histological evaluation of salivary gland biopsies was performed. Liquid chromatography-mass spectrometry (LC-MS) was conducted on tear fluid and stimulated whole saliva, and proteomic biomarker profiles were generated. Extracellular vesicle (EVs) isolation and characterisation from both fluids were also combined with LC-MS. The LC-MS data were analysed for quantitative differences between patient and control groups using Scaffold. Database for Annotation, Visualization and Integrated Discovery (DAVID) and Functional Enrichment Analysis Tool (FunRich) w...Continue Reading

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Citations

Feb 18, 2020·Biomarkers in Medicine·Maria C EdmanSarah F Hamm-Alvarez
Dec 31, 2019·Disease Markers·Snezana Agatonovic-KustrinGalina Ramenskaya
Jul 28, 2020·International Journal of Molecular Sciences·Lucía Melguizo-RodríguezRebeca Illescas-Montes
Dec 15, 2020·Journal of Chromatography. a·Thanaporn LiangsupreeMarja-Liisa Riekkola
Jan 23, 2021·Expert Review of Clinical Immunology·Loukas ChatzisAndreas V Goules
Jan 28, 2021·International Journal of Molecular Sciences·Jeong-Sun HanDong-Jun Lim
Dec 4, 2020·International Journal of Molecular Sciences·Xianglan ZhangEunae Sandra Cho
Dec 17, 2020·Eye & Contact Lens·Cem Simsek, Murat Dogru
Jun 3, 2021·International Journal of Molecular Sciences·Francesco FinamoreChiara Baldini
Jun 25, 2021·Frontiers in Medicine·Claudio VitaliNicoletta Del Papa
Sep 14, 2021·Immunity, Inflammation and Disease·Mingde LiXingxing Huo

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Methods Mentioned

BETA
biopsies
biopsy
size-exclusion chromatography
flow cytometry
ubiquitination

Software Mentioned

ProteoWizard
FunRich
Proteome
Scaffold
Xcalibur
DAVID
NTA
Mascot house
Functional Enrichment Analysis Tool ( FunRich
Mascot

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