Proteomic and immunomic analysis of Schistosoma mekongi egg proteins

Experimental Parasitology
Tipparat ThiangtrongjitOnrapak Reamtong

Abstract

Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antigens are important in circumoval precipitin tests (COPTs) and other diagnostic techniques. Little is known regarding the egg proteins of S. mekongi, and so we applied immunoblotting and mass spectrometry-based proteomic approaches to study these proteins and their antigenicity. A total of 360 unique proteins were identified in S. mekongi eggs using proteomic analyses. The major protein components of S. mekongi eggs were classified into several groups by functions, including proteins of unknown function, structural proteins, and regulators of transcription and translation. The most abundant proteins in S. mekongi eggs were antioxidant proteins, potentially reflecting the need to neutralize reactive oxidative species released from host immune cells. Immunomic analyses revealed that only DNA replication factor Cdt1 and heat shock protein 70 overlap between the proteins recognized by sera of infected mice and humans, il...Continue Reading

Citations

Jun 6, 2019·Parasite : Journal De La Société Française De Parasitologie·Supaporn NuamtanongPoom Adisakwattana
Jul 12, 2019·Scientific Reports·Nattapon SimanonOnrapak Reamtong
Apr 3, 2020·PLoS Neglected Tropical Diseases·Porntida KobpornchaiPoom Adisakwattana
Apr 4, 2021·Pathogens·Adam P S Bennett, Mark W Robinson
Aug 31, 2021·Frontiers in Molecular Biosciences·David B PirovichPatrick J Skelly

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