Proteomic and network analysis of proteins regulated by REX1 in human embryonic stem cells

Proteomics
Mi-Young SonYee Sook Cho

Abstract

Recent studies have suggested that REX1 (reduced expression 1) plays an important role in pluripotency, proliferation, and differentiation. However, the molecular mechanisms involved in REX1-dependent regulation of diverse cellular processes remain unclear. To elucidate the regulatory functions of REX1 in human embryonic stem cells (hESCs), comparative proteomic analysis was performed on REX1 RNAi specifically silenced hESCs. Analysis of the proteome via nano-LC-MS/MS identified 140 differentially expressed proteins (DEPs) displaying a >2-fold difference in expression level between control and REX1 knockdown (KD) hESCs, which were then compared with transcriptome data and validated by quantitative real-time RT-PCR and Western blotting. These DEPs were analyzed by GO, pathway, and functional clustering analyses to determine the molecular functions of the proteins and pathways regulated by REX1. The REX1 KD-mediated DEPs mapped to major biological processes involved in the regulation of ribosome-mediated translation and mitochondrial function. Functional network analysis revealed a highly interconnected network among these DEPs and indicated that these interconnected proteins are predominantly involved in translation and the regu...Continue Reading

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Citations

Jun 24, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dae-Soo KimHyun-Soo Cho
Sep 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kwang Bo JungMi-Young Son
Dec 6, 2017·International Journal of Molecular Medicine·Jung-Hwa OhMi-Young Son

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