Proteomic and Post-Translational Modification Profiling of Exosome-Mimetic Nanovesicles Compared to Exosomes

Proteomics
Amirmohammad Nasiri KenariAndrew F Hill

Abstract

Issues associated with upscaling exosome production for therapeutic use may be overcome through utilizing artificial exosomes. Cell-derived mimetic nanovesicles (M-NVs) are a potentially promising alternative to exosomes for clinical applicability, demonstrating higher yield without incumbent production and isolation issues. Although several studies have shown that M-NVs have similar morphology, size and therapeutic potential compared to exosomes, comprehensive characterization and to what extent M-NVs components mimic exosomes remain elusive. M-NVs were generated through the extrusion of cells and proteomic profiling demonstrated an enrichment of proteins associated with membrane and cytosolic components. The proteomic data herein reveal a subset of proteins that are highly abundant in M-NVs in comparison to exosomes. M-NVs contain proteins that largely represent the parental cell proteome, whereas the profile of exosomal proteins highlight their endosomally derived origin. This advantage of M-NVs alleviates the necessity of endosomal sorting of endogenous therapeutic proteins or RNA into exosomes. This study also highlights differences in protein post-translational modifications among M-NVs, as distinct from exosomes. Overall...Continue Reading

References

Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Noah E Robinson
Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Oct 21, 2004·Mass Spectrometry Reviews·Christian Schöneich
Aug 9, 2005·Journal of Proteome Research·Ji-Eun KimForest M White
Jan 4, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robyn A SharplesAndrew F Hill
Feb 8, 2008·Molecular & Cellular Proteomics : MCP·Mitsunori FukudaTakashi Itoh
Jun 24, 2008·Protein Science : a Publication of the Protein Society·Takumi TakataKirsten J Lampi
Jul 9, 2008·World Journal of Gastroenterology : WJG·Henning Schulze-BergkamenMarkus Moehler
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 28, 2009·Nature·Camilla Raiborg, Harald Stenmark
May 22, 2009·Analytical Biochemistry·Da RenPavel V Bondarenko
Jun 6, 2009·Nature Reviews. Immunology·Clotilde ThéryElodie Segura
Jun 16, 2009·Traffic·Meng-Tse Gabe LeeDavid G Lambright
Apr 27, 2010·Journal of Proteome Research·Jacek R WiśniewskiMatthias Mann
Jul 20, 2010·Nature Biotechnology·Guoqiang XuSamie R Jaffrey
Mar 23, 2011·Nature Biotechnology·Lydia Alvarez-ErvitiMatthew J A Wood
Mar 25, 2011·Biochemical Society Transactions·Sanyukta Rana, Margot Zöller
Jul 19, 2011·Developmental Cell·William M HenneScott D Emr
Sep 6, 2011·Molecular & Cellular Proteomics : MCP·Sebastian A WagnerChunaram Choudhary
Dec 31, 2011·Molecules and Cells·Yun Soo BaeYoung Do Yoo
Feb 23, 2012·Cold Spring Harbor Perspectives in Medicine·Leonidas Stefanis
Jun 5, 2012·Cell·Alfredo CastelloMatthias W Hentze
Jul 25, 2012·Cell Death & Disease·O Marques, T F Outeiro
Sep 21, 2013·Nature Protocols·Namrata D UdeshiSteven A Carr
Dec 3, 2013·Journal of Proteome Research·Angelito I NepomucenoDavid C Muddiman
Apr 29, 2014·Seminars in Cancer Biology·Carolina Villarroya-BeltriMaría Mittelbrunn
Jul 6, 2014·Biosensors & Bioelectronics·Liang WangXiyun Guan
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Feb 28, 2015·Seminars in Cell & Developmental Biology·Katalin Szabó-TaylorEdit I Buzás
May 1, 2015·Journal of Proteome Research·Simion KreimerAlexander R Ivanov
May 7, 2015·Oncotarget·Shivakumar KeerthikumarSuresh Mathivanan
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Nov 21, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Myung Soo KimElena V Batrakova
Jan 5, 2016·Journal of Extracellular Vesicles·Thomas LenerBernd Giebel

❮ Previous
Next ❯

Citations

Jul 9, 2020·International Journal of Molecular Sciences·Susannah HallalKimberley Louise Alexander-Kaufman
Sep 10, 2020·Journal of Peptide Science : an Official Publication of the European Peptide Society·Laura Sršan, Thomas Ziegler
May 16, 2019·Proteomes·Magdalena SurmanMałgorzata Przybyło
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tatiana S GolubevaKonstantin E Orishchenko
Jan 13, 2021·Journal of Nanobiotechnology·Lei CaoLei Wang
Dec 31, 2020·International Journal of Molecular Sciences·Hyosuk KimYoosoo Yang
Jul 3, 2021·Cells·Cherrie D ShermanSusmita Sahoo
Aug 14, 2021·Journal of Nanobiotechnology·Yong-Jiang LiDa-Xiong Xiang
Aug 24, 2021·Advanced Therapeutics·Stephanie M KronstadtSteven M Jay
Aug 28, 2021·International Journal of Molecular Sciences·Yichen JuLiying Zhang
Aug 31, 2021·ACS Biomaterials Science & Engineering·Xuemei JiaDayong Yang
Jul 27, 2021·Expert Review of Molecular Diagnostics·Raheleh HeydariAnna Meyfour

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.