Proteomic and transcriptomic analysis to unravel the influence of high temperature on banana fruit during postharvest storage

Functional & Integrative Genomics
Taotao LiYueming Jiang

Abstract

Banana, an important food, incurs significant economic losses due to high storage temperature. Integrative analysis of proteome and transcriptome profiles of the banana peel stored at 20 °C (control) and 30 °C (HT) was used to investigate the molecular mechanism in response to high temperature stress. Critical proteins and genes relating to the response of banana fruit to HT stress were evaluated using partial least squares-discriminant analysis (PLS-DA) and orthogonal signal correction partial least squares-discriminant analysis (OPLS-DA). HT stress influenced proteins/genes related to chlorophyll metabolism, fruit firmness, signal transduction, energy metabolism, and stress response and defense. Together with scanning electron microscopy (SEM) and real time quantitative PCR (RT-qPCR) results, it can be concluded that HT stress resulted in stay-green ripening of banana fruit. Additionally, HT stress accelerated firmness loss and senescence of banana peel, might mainly through regulating hormone signaling pathway, stress protective ability, and energy metabolism in the banana peel. Our study provided a clearer understanding of regulatory mechanisms of HT treatment on banana fruit and potential genetic resources for the improvem...Continue Reading

References

Apr 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laigeng LiVincent L Chiang
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Mar 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·S P Burg, K V Thimann
Nov 8, 2006·Plant, Cell & Environment·Mingxiang LiangYajun Wu
Jan 24, 2007·The Plant Cell·Adriana PruzinskáStefan Hörtensteiner
Oct 23, 2008·Journal of Experimental Botany·Maria Rapala-KozikKatarzyna Ostrowska
Feb 18, 2009·The Plant Journal : for Cell and Molecular Biology·Louise F ThatcherKemal Kazan
Aug 25, 2009·Journal of Experimental Botany·Xiaotang YangZhaoqi Zhang
Dec 17, 2009·The Biochemical Journal·Thomas A DeFalcoWayne A Snedden
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Jan 17, 2012·Proteomics·Wilson W B GohLimsoon Wong
May 1, 2012·Molecular & Cellular Proteomics : MCP·Niclas OlssonChrister Wingren
May 18, 2012·Metabolomics : Official Journal of the Metabolomic Society·Ewa SzymańskaJohan A Westerhuis
Dec 17, 2016·Food Chemistry·Kalynka Gabriella do LivramentoLuciano Vilela Paiva
Jan 29, 2017·Annals of Botany·Nancy Eunice Niño CastañedaRobert Neil Gerard Miller
Apr 10, 2017·Photosynthesis Research·Alexander G IvanovNorman P A Huner

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
scanning electron microscopy
electrophoresis
PCR
RNA-Seq
protein folding
MDA
environmental thermal stress

Software Mentioned

[UNK] Basic 2 - D Gel Analysis
BLAST
PLS
Blast2Go
OPLS
DA
SPSS
SIMCA
PDQuest
Mascot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.