Proteomic approach to the identification of novel delta-lactoferrin target genes: Characterization of DcpS, an mRNA scavenger decapping enzyme

Biochimie
Christophe MarillerAnnick Pierce

Abstract

The expression of the transcription factor DeltaLf is deregulated in cancer cells. Its overexpression provokes cell cycle arrest along with antiproliferative effects and we recently showed that the Skp1 gene promoter was a target of DeltaLf. Skp1 belongs to the Skp1/Cullin-1/F-box ubiquitin ligase complex responsible for the ubiquitination and the proteosomal degradation of numerous cellular regulators. The transcriptional activity of DeltaLf is highly controlled and negatively regulated by O-GlcNAc, a dynamic post-translational modification known to regulate the functions of many intracellular proteins. We, therefore, constructed a DeltaLf-M4 mutant corresponding to a constitutively active DeltaLf isoform in which all the glycosylation sites were mutated. In order to discover novel targets of DeltaLf transcriptional activity and to investigate the impact of the O-GlcNAc regulation on this activity in situ we compared the proteome profiles of DeltaLf- and DeltaLf-M4-expressing HEK293 cells versus null plasmid transfected cells. A total of 14 differentially expressed proteins were visualized by 2D electrophoresis and silver staining and eight proteins were identified by mass spectrometry analyses (MALDI-TOF; LC-MS/MS), all of wh...Continue Reading

Citations

May 5, 2009·Médecine sciences : M/S·Annick PierceJoël Mazurier
Apr 21, 2010·The Journal of Biological Chemistry·Stéphan HardivilléAnnick Pierce
Feb 11, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christophe MarillerAnnick Pierce
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yalin LiaoBo Lönnerdal
May 16, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Stéphan HardivilléAnnick Pierce
Feb 16, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Esthelle HoedtAnnick Pierce
Aug 1, 2013·Biochemical and Biophysical Research Communications·Zhou FengZhang Lianwen
Feb 15, 2011·Biochimica Et Biophysica Acta·Juliette MartinJean-François Dufour
Jul 5, 2013·Molecular & Cellular Proteomics : MCP·Hai-Bin RuanXiaoyong Yang

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