Proteomic biomarkers in mid-trimester amniotic fluid associated with adverse pregnancy outcomes in patients with systemic lupus erythematosus

PloS One
Hae Sun JeonJong Kwan Jun

Abstract

We aimed to explore the proteomic profiles of mid-trimester amniotic fluid in pregnant women with systemic lupus erythematosus (SLE) according to the occurrence of adverse pregnancy outcome (APO). The study population included 35 pregnant women with SLE who underwent clinically indicated amniocentesis at 15-24 weeks of gestation. Patients were divided into two groups according to pregnancy outcomes: SLE patients without APO (Group 1) and SLE patients with APO (Group 2). Stored samples of amniotic fluid were analyzed using mass spectrometry (MS)-based proteomics with two-step approach, consisting of discovery and verification phase. In the discovery phase, 44 proteins were differentially expressed between Group 1 and Group 2. In the verification phase, differentially expressed proteins (DEPs) were verified in independent samples using DIA method. Four proteins including filamin A (FLNA), sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 (SVEP1), lecithin-cholesterol acyltransferase (LCAT), and transglutaminase 2 (TGM2) were differentially expressed both in discovery and verification phase. To select the best combination of proteins for discriminating two groups, three-fold cross validation (CV) with repe...Continue Reading

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Citations

Oct 14, 2021·Database : the Journal of Biological Databases and Curation·Dan ShaoJuan Cui
Nov 7, 2021·Clinical and Experimental Medicine·Ying XingJing Ma

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Methods Mentioned

BETA
proteomic profiling
enzyme-linked immunosorbent assay

Software Mentioned

Perseus
Statistical Package for the Social Sciences ( SPSS )
PROMISSE
MaxQuant
R
Spectronaut

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