Proteomic Characterization of Antibiotic Resistance, and Production of Antimicrobial and Virulence Factors in Streptococcus Species Associated with Bovine Mastitis. Could Enzybiotics Represent Novel Therapeutic Agents Against These Pathogens?

Antibiotics
Ana G AbrilTomás G Villa

Abstract

Streptococcus spp. are major mastitis pathogens present in dairy products, which produce a variety of virulence factors that are involved in streptococcal pathogenicity. These include neuraminidase, pyrogenic exotoxin, and M protein, and in addition they might produce bacteriocins and antibiotic-resistance proteins. Unjustifiable misuse of antimicrobials has led to an increase in antibiotic-resistant bacteria present in foodstuffs. Identification of the mastitis-causing bacterial strain, as well as determining its antibiotic resistance and sensitivity is crucial for effective therapy. The present work focused on the LC-ESI-MS/MS (liquid chromatography-electrospray ionization tandem mass spectrometry) analysis of tryptic digestion peptides from mastitis-causing Streptococcus spp. isolated from milk. A total of 2706 non-redundant peptides belonging to 2510 proteins was identified and analyzed. Among them, 168 peptides were determined, representing proteins that act as virulence factors, toxins, anti-toxins, provide resistance to antibiotics that are associated with the production of lantibiotic-related compounds, or play a role in the resistance to toxic substances. Protein comparisons with the NCBI database allowed the identific...Continue Reading

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Citations

Sep 19, 2020·Antibiotics·Tomás G Villa, Carmen Sieiro
Jul 3, 2021·Proteomes·Eleni I KatsarouAngeliki I Katsafadou
Jul 24, 2021·Journal of Environmental Management·Sakina BombaywalaNishant A Dafale
Aug 8, 2021·International Journal of Molecular Sciences·Ana G AbrilTomás G Villa

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Methods Mentioned

BETA
acetylation

Software Mentioned

Basic Local Alignment Tool for proteins ( BLASTp )
SEQUEST
Basic Local Alignment Search Tool for proteins ( BLASTp )
Proteome Discoverer
BLASTp
HT

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