Proteomic characterization of donkey milk "caseome"

Journal of Chromatography. a
Lina ChianeseLuigi Ramunno

Abstract

At present, compared with bovine milk, the characterization of donkey milk caseins is at a relatively early stage progress, and only limited data are related to its genetic polymorphism. In this work, the heterogeneity of donkey caseome was investigated using a proteomic approach, based on one- (PAGE, UTLIEF) and two-dimensional (PAGE-->UTLIEF) electrophoresis, stained with either Coomassie Brilliant Blue or specific polyclonal antibodies, and structural MS analysis. These combined methodologies allowed the contemporary identification of donkey alpha(s1), alpha(s2), beta and kappa-CN with their related heterogeneity due to phosphorylation (alpha(s1), alpha(s2) and beta-CN), glycosylation (kappa-CN) and incorrect splicing of RNA in mRNA (deleted forms of alpha(s1)-CN and beta-CN). The results achieved showed 11 components for kappa-CN, six phosphorylated components for beta and alpha(s1)-CN and three main phosphorylated components for alpha(s2)-CN, each accounting for 10, 11 and 12 P/mole. At this regard, for the first time, the primary structure of the expressed protein corresponding to the only available donkey alpha(s2)-CN cDNA sequence was determined. Furthermore beta-CN was found in homozygous and heterozygous state for the...Continue Reading

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Citations

Feb 19, 2013·Journal of Agricultural and Food Chemistry·Therese Uniacke-LoweDaniel M Mulvihill
Jun 8, 2011·Journal of Proteomics·Vincenzo CunsoloSalvatore Foti
May 30, 2012·Journal of Proteomics·Paola RoncadaLuigi Bonizzi
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Rosaria SalettiSalvatore Foti
Feb 11, 2015·Journal of Proteomics·Susy PiovesanaAldo Laganà
Mar 10, 2017·International Journal of Food Sciences and Nutrition·Mei YangJunrui Wu
Oct 19, 2011·European Journal of Mass Spectrometry·Vincenzo CunsoloSalvatore Foti
Jun 21, 2018·The Journal of Dairy Research·Michele FacciaAngela G D'Alessandro
May 1, 2019·The Journal of Dairy Research·Gianfranco CosenzaAlfredo Pauciullo
Sep 30, 2019·Journal of Dairy Science·A G D'AlessandroM Faccia
Jul 30, 2020·Animals : an Open Access Journal From MDPI·Michele FacciaYonas Hailu
Dec 28, 2019·Foods·Michele FacciaAngela Gabriella D'Alessandro
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Shanshan LiJianping Wu
Aug 12, 2021·The Journal of Dairy Research·Angela Gabriella D'AlessandroMichele Faccia
Jun 4, 2014·Journal of Agricultural and Food Chemistry·Rosa Pizzano, Elisabetta Salimei

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