Proteomic characterization of EL4 lymphoma-derived tumors upon chemotherapy treatment reveals potential roles for lysosomes and caspase-6 during tumor cell death in vivo

Proteomics
David A KramerRichard P Fahlman

Abstract

The murine mouse lymphoblastic lymphoma cell line (EL4) tumor model is an established in vivo apoptosis model for the investigation of novel cancer imaging agents and immunological treatments due to the rapid and significant response of the EL4 tumors to cyclophosphamide and etoposide combination chemotherapy. Despite the utility of this model system in cancer research, little is known regarding the molecular details of in vivo tumor cell death. Here, we report the first in-depth quantitative proteomic analysis of the changes that occur in these tumors upon cyclophosphamide and etoposide treatment in vivo. Using a label-free quantitative proteomic approach a total of 5838 proteins were identified in the treated and untreated tumors, of which 875 were determined to change in abundance with statistical significance. Initial analysis of the data reveals changes that may have been predicted, such as the downregulation of ribosomes, but demonstrates the robustness of the dataset. Analysis of the dataset also reveals the unexpected downregulation of caspase-3 and an upregulation of caspase-6 in addition to a global upregulation of lysosomal proteins in the bulk of the tumor.

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Citations

May 17, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ramanaguru S PiragasamRichard P Fahlman
Nov 19, 2019·Protein and Peptide Letters·Mohamed A. EldeebFaraz Hussein
Aug 21, 2020·Anti-cancer Agents in Medicinal Chemistry·Mohamed A Eldeeb
May 3, 2020·Anti-cancer Agents in Medicinal Chemistry·Mohamed A RaghebAbdel-Hady A Abdel-Wahab
May 18, 2020·Anti-cancer Agents in Medicinal Chemistry·Marwa H SolimanAbdel-Hady A Abdel-Wahab

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