Proteomic characterization of herbicide safener-induced proteins in the coleoptile of Triticum tauschii seedlings

Proteomics
Qin Zhang, Dean E Riechers

Abstract

Proteomic methods such as two-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry, as well as immunoblotting, were used to identify herbicide safener-induced proteins in the coleoptile of Triticum tauschii, a diploid wheat containing the D genome also found in the cultivated, hexaploid wheat Triticum aestivum. The herbicide safener fluxofenim dramatically increased protein abundance in the molecular weight (M(r)) range of 24 to 30 kDa, as well as a few higher M(r) proteins, in the coleoptile of T. tauschii seedlings. In total, twenty proteins were identified in this study. Eleven proteins were highly safener induced and only weakly expressed in the control; seven proteins were new safener induced proteins that were not detected in the control. Two other proteins were constitutively expressed in both the control and safener-treated coleoptiles. Among the eighteen inducible proteins, fifteen were glutathione S-transferase (GST) subunits that fall into three subclasses: eight proteins were from the tau subclass, six proteins were from the phi subclass, and one protein was from the lambda class. Another three safener inducible proteins showed homology to the aldo/keto reductase family and with protein...Continue Reading

Citations

Jul 23, 2011·The Journal of Biological Chemistry·Mark SkipseyRobert Edwards
Oct 11, 2012·Omics : a Journal of Integrative Biology·Khalid Rehman HakeemMunir Ozturk
Jan 5, 2006·Genome Génome / Conseil National De Recherches Canada·Wenchun ZhouDean E Riechers
Dec 13, 2006·Trends in Plant Science·Joshua S YuanC Neal Stewart
Jun 16, 2005·Mass Spectrometry Reviews·Ganesh Kumar Agrawal, Randeep Rakwal
Aug 28, 2007·Proteomics·Dong-Gi LeeByung-Hyun Lee
Mar 18, 2006·Proteomics·Juan López-Barea, José Luis Gómez-Ariza
Feb 24, 2006·Phytochemistry·Ian CumminsRobert Edwards
Jul 14, 2017·Environmental Geochemistry and Health·Mihiri SeneviratneMeththika Vithanage
Mar 30, 2021·Frontiers in Plant Science·Gulab RanganiNilda Roma-Burgos

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