Proteomic characterization of isolated retinal pigment epithelium microvilli.

Molecular & Cellular Proteomics : MCP
V L BonilhaJoe G Hollyfield

Abstract

Polarized epithelial cells are characterized by displaying compartmentalized functions associated with differential distribution of transporters, structural proteins, and signaling molecules on their apical and basolateral surfaces. Their apical surfaces frequently elaborate microvilli, which vary in structure according to the specific type and function of each epithelium. The molecular basis of this heterogeneity is poorly understood. However, differences in function will undoubtedly be reflected in the specific molecular composition of the apical surface in each epithelial subtype. We have exploited a method for isolating microvilli from the mouse eye using wheat germ agglutinin (WGA)-agarose beads to begin to understand the specific molecular composition of apical microvilli of the retinal pigment epithelium (RPE) and expand our knowledge of the potential function of this interface. Initially, apical RPE plasma membranes bound to WGA beads were processed for morphological analysis using known apical and basolateral surface markers. The protein composition of the apical microvilli was then established using proteomic analysis. Over 200 proteins were identified, including a number of proteins previously known to be localized t...Continue Reading

References

Dec 2, 1991·Mechanisms of Ageing and Development·P NapoleoneF Amenta
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S I HarikG Perry
Aug 18, 1989·Science·E Rodriguez-Boulan, W J Nelson
Mar 1, 1987·Experimental Eye Research·D K Vaughan, S K Fisher
Feb 1, 1985·Archives of Disease in Childhood·A D PhillipsJ A Walker-Smith
Jan 1, 1984·The Journal of Membrane Biology·W Almers, C Stirling
Aug 1, 1995·Current Opinion in Cell Biology·T Hasson, M S Mooseker
Feb 1, 1995·Differentiation; Research in Biological Diversity·V HuotariS Eskelinen
Sep 1, 1994·The Histochemical Journal·K E MuseL W Oberley
Jan 1, 1993·Journal of Cell Science. Supplement·D Bok
Apr 1, 1993·The Journal of Cell Biology·D GundersenE Rodriguez-Boulan
Oct 1, 1995·Journal of Biochemistry·T MiyauchiT Muramatsu
Jan 1, 1995·Ophthalmic Research·I Weisse
Dec 1, 1996·Neuropathology and Applied Neurobiology·T HiraiC Itakura
Nov 25, 1997·Proceedings of the National Academy of Sciences of the United States of America·S C FinnemannE Rodriguez-Boulan
Dec 31, 1997·The Journal of Biological Chemistry·S YingW W Kao
Jan 26, 1999·Annals of the New York Academy of Sciences·A D MarmorsteinE Rodriguez-Boulan
Dec 30, 1999·The Journal of Cell Biology·V L BonilhaE Rodriguez-Boulan
Jul 27, 2001·Mechanisms of Ageing and Development·D L SchmuckerR L Owen
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Basil HanssPaul E Klotman
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Yumiko MizukawaTetsuro Urushidani
Oct 23, 2002·Proceedings of the National Academy of Sciences of the United States of America·John W CrabbJoe G Hollyfield
Feb 26, 2003·Molecular & Cellular Proteomics : MCP·Karen A WestJohn W Crabb
Apr 12, 2003·The Biochemical Journal·Gert H HansenE Michael Danielsen
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Jean-Marie SerotGilbert C Faure
Apr 30, 2003·Parasitology Research·Sung-Jong HongJae-Ran Ryu
May 27, 2003·Investigative Ophthalmology & Visual Science·Shukti ChakravartiDavid E Birk
Jan 28, 2004·Investigative Ophthalmology & Visual Science·Maria NawrotJohn C Saari
Sep 1, 2004·Experimental Eye Research·Vera L BonilhaJohn W Crabb

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Citations

Jul 10, 2009·Molecular Biology of the Cell·Ah-Lai LawMatthew J Hayes
Jun 16, 2012·PloS One·Xiaorong GuVera L Bonilha
May 27, 2009·International Journal of Molecular Sciences·Petras Dzeja, Andre Terzic
Dec 15, 2015·The Protein Journal·Srinivas R SripathiWan Jin Jahng
Dec 21, 2007·Proteomics·Marcin BalcerzakSlawomir Pikula
Nov 30, 2007·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Thomas C LamChi-Ho To
Nov 18, 2005·Experimental Eye Research·Vera L BonilhaJoe G Hollyfield
Jun 4, 2013·Neurobiology of Aging·Michael R R BöhmSolon Thanos
Oct 5, 2013·Experimental Eye Research·Vera L Bonilha
Sep 12, 2012·Ophthalmic Research·Antonis KoutsonasAndreas W A Weinberger
Nov 1, 2016·Scientific Reports·Kedsarin Fong-Ngern, Visith Thongboonkerd
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·P Krishnamurthy, J D Schuetz
Oct 24, 2007·Cancer Investigation·Jeffrey M Skolnik, Peter C Adamson
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S KoshibaT Ishikawa
Jul 19, 2014·Drug Metabolism Reviews·Milena Julia SzafraniecLeszek Fiedor
Jan 24, 2006·The Journal of Biological Chemistry·Martin Lidén, Ulf Eriksson
Sep 14, 2019·Journal of Cell Science·Chen YuSilvia C Finnemann
Dec 1, 2010·The Journal of Veterinary Medical Science·Safwat Ali Mohamed AliMasato Uehara
Aug 29, 2020·Oxidative Medicine and Cellular Longevity·Camille Keisha MahendraBey Hing Goh
Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Karina E GuziewiczGustavo D Aguirre

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