Proteomic characterization of postmortem amyloid plaques isolated by laser capture microdissection.

The Journal of Biological Chemistry
Lujian LiaoJunmin Peng

Abstract

The presence of amyloid plaques in the brain is one of the pathological hallmarks of Alzheimer's disease (AD). We report here a comprehensive proteomic analysis of senile plaques from postmortem AD brain tissues. Senile plaques labeled with thioflavin-S were procured by laser capture microdissection, and their protein components were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified a total of 488 proteins co-isolated with the plaques, and we found multiple phosphorylation sites on the neurofilament intermediate chain, implicating the complexity and diversity of cellular processes involved in the plaque formation. More significantly, we identified 26 proteins enriched in the plaques of two AD cases by quantitative comparison with surrounding non-plaque tissues. The localization of several proteins in the plaques was further confirmed by the approach of immunohistochemistry. In addition to previously identified plaque constituents, we discovered novel association of dynein heavy chain with the plaques in human postmortem brain and in a double transgenic AD mouse model, suggesting that neuronal transport may play a role in neuritic degeneration. Overall, our results revealed for the first time...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Lichtenberg-KraagE Mandelkow
Apr 1, 1990·Neurology·T I Mandybur, C C Chuirazzi
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C L MastersK Beyreuther
May 16, 1984·Biochemical and Biophysical Research Communications·G G Glenner, C W Wong
May 3, 1996·Neuroscience Letters·R LayfieldR J Mayer
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Dec 24, 1997·Proceedings of the Society for Experimental Biology and Medicine·K Kopec, J P Chambers
Jul 24, 1998·Trends in Genetics : TIG·N L SimoneL A Liotta
Aug 13, 1998·Journal of Neural Transmission. Supplementum·R D Terry
Nov 12, 1998·The American Journal of Pathology·A KinoshitaJ Kimura
Jul 15, 1999·Nature Biotechnology·A J LinkJ R Yates
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·M K AhlijanianJ D McNeish
Jun 7, 2000·The Journal of Biological Chemistry·M HashiguchiH K Paudel
Jun 20, 2000·Neurobiology of Aging·H AkiyamaT Wyss-Coray
Feb 24, 2001·Journal of Neuropathology and Experimental Neurology·E LevyF Tagliavini
Aug 7, 2001·Journal of Neuropathology and Experimental Neurology·A NunomuraM A Smith
Aug 25, 2001·Annual Review of Neuroscience·V M LeeJ Q Trojanowski
Sep 11, 2001·The American Journal of Pathology·A DengG W Rebeck
Dec 18, 2001·Journal of Mass Spectrometry : JMS·J Peng, S P Gygi
Jan 16, 2002·Current Biology : CB·Jens S AndersenAngus I Lamond
Feb 20, 2002·Trends in Biochemical Sciences·Juri Rappsilber, Matthias Mann
Jun 27, 2002·Nature Neuroscience·Philip C WongDonald L Price
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Oct 3, 2002·The EMBO Journal·Yuzuru ShiioRobert N Eisenman
Oct 24, 2002·Neurobiology of Aging·Gianluigi ForloniAlessandro Bertoli
Dec 5, 2002·Neurochemical Research·T TsujiS Shimohama

❮ Previous
Next ❯

Citations

Sep 7, 2007·Acta Neuropathologica·John C Fiala
Nov 6, 2008·Acta Neuropathologica·Thibaud LebouvierCharles Duyckaerts
Mar 5, 2009·Acta Neuropathologica·Maria ZellnerEllen Umlauf
Jul 4, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Thomas J MontineJing Zhang
May 31, 2008·Journal of Proteome Research·Qiangwei XiaJunmin Peng
Sep 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bing BaiJunmin Peng
Feb 1, 2011·The Journal of Biological Chemistry·Eric B DammerJunmin Peng
Dec 19, 2009·Molecular & Cellular Proteomics : MCP·Faneng Sun, Valeria Cavalli
Dec 30, 2011·Neuro-degenerative Diseases·Konstantin Glebov, Jochen Walter
Feb 5, 2010·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Oct 12, 2010·Molecular Neurodegeneration·Chera L MaaroufAlex E Roher
Aug 17, 2010·PLoS Biology·Della C DavidCynthia Kenyon
Jul 21, 2012·PloS One·Dapeng LiangHongxing Lei
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Qiangwei XiaJunmin Peng
May 4, 2006·The Journal of Clinical Psychiatry·Andreas PapassotiropoulosEric M Reiman
Mar 22, 2014·Journal of Proteome Research·Surendra DasariAhmet Dogan
Oct 19, 2006·Journal of Biomedicine & Biotechnology·Marie W WootenMichael C Wooten
Feb 27, 2014·Bioanalysis·David Touboul, Mathieu Gaudin
Apr 12, 2007·Expert Review of Proteomics·Simon LovestoneMalcolm Ward
Nov 26, 2005·Expert Review of Proteomics·Oliver SchmidtMichael Hamacher
May 1, 2008·Expert Opinion on Medical Diagnostics·Steven Pelech
Jul 26, 2006·Expert Opinion on Drug Metabolism & Toxicology·Frank A Witzmann, Matthew R Richardson
Sep 19, 2008·Journal of Neuropathology and Experimental Neurology·Jing ZhangThomas J Montine
Nov 21, 2009·Drug and Alcohol Dependence·Melinda E LullKent E Vrana
Mar 3, 2009·Neurobiology of Disease·Pier Giorgio MastroberardinoJ Timothy Greenamyre
Nov 30, 2006·Drug Discovery Today·Andrew Lockhart
Mar 13, 2015·Neuropathology and Applied Neurobiology·Janet van EerselLars M Ittner
Apr 26, 2006·Basic & Clinical Pharmacology & Toxicology·Mollisa M ElrickDavid C Thompson
Apr 11, 2007·Journal of Neurochemistry·Jose A Morón, Lakshmi A Devi
Mar 29, 2007·Brain Pathology·James B LeverenzJing Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.