Proteomic characterization of the striatum and midbrain treated with 6-hydroxydopamine: alteration of 58-kDa glucose-regulated protein and C/EBP homologous protein

Free Radical Research
Yoko Ogawa AkazawaEtsuo Niki

Abstract

The present study performed proteomic analysis of the midbrain and striatum of 6-hydroxydopamine (6-OHDA)-treated neonatal rats--a model of attention-deficit hyperactivity disorder (ADHD). Proteomic analysis revealed that a 58-kDa glucose-regulated protein (Grp58) was temporarily phosphorylated and its level was elevated by 6-OHDA. Furthermore, 6-OHDA increased the expression level of C/EBP homologous protein (CHOP), a mediator of endoplasmic reticulum (ER) stress response, in the midbrain and striatum. In vitro experiments using PC12 cells revealed that 6-OHDA or hydrogen peroxide could induce the elevation of Grp58 and CHOP. 6-OHDA could induce the elevation of Grp58 and CHOP in the presence of catalase, a hydrogen peroxide-removing enzyme, suggesting that the elevation of Grp58 and CHOP are induced by both hydrogen peroxide and p-quinone generated by 6-OHDA. Collectively, these findings suggest that ER stress involving the alteration of Grp58 and CHOP play a significant role in the induction of insults by 6-OHDA in vivo.

References

Apr 1, 1977·Journal of Medicinal Chemistry·Y O LiangR N Adams
Jun 20, 2000·FEBS Letters·F Antunes, E Cadenas
Sep 13, 2000·Journal of Molecular Neuroscience : MN·J C GarcíaR González
Jan 30, 2002·The Journal of Biological Chemistry·Pravin B SehgalKirit Patel
Feb 25, 2003·The Journal of Biological Chemistry·William Andrew Holtz, Karen Laurel O'Malley
Jun 24, 2003·Rapid Communications in Mass Spectrometry : RCM·Hiroki KuyamaOsamu Nishimura
Sep 24, 2004·Journal of Neurochemistry·Yoshinori MasuoEtsuo Niki
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xinhe WangJiyan Ma
May 8, 2007·FEBS Letters·Min Ni, Amy S Lee
Jun 15, 2007·Journal of Proteome Research·Misato HiranoYoshinori Masuo
Oct 30, 2007·Biochemical and Biophysical Research Communications·Yoshiko TokutomiShokei Kim-Mitsuyama
Apr 4, 2008·Journal of Neurochemistry·April A DukesTeresa G Hastings

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