Proteomic comparison of different synaptosome preparation procedures.

Amino Acids
Péter GulyássyKatalin Adrienna Kékesi

Abstract

Synaptosomes are frequently used research objects in neurobiology studies focusing on synaptic transmission as they mimic several aspects of the physiological synaptic functions. They contain the whole apparatus for neurotransmission, the presynaptic nerve ending with synaptic vesicles, synaptic mitochondria and often a segment of the postsynaptic membrane along with the postsynaptic density is attached to its outer surface. As being artificial functional organelles, synaptosomes are viable for several hours, retain their activity, membrane potential, and capable to store, release, and reuptake neurotransmitters. Synaptosomes are ideal subjects for proteomic analysis. The recently available separation and protein detection techniques can cope with the reduced complexity of the organelle and enable the simultaneous qualitative and quantitative analysis of thousands of proteins shaping the structural and functional characteristics of the synapse. Synaptosomes are formed during the homogenization of nervous tissue in the isoosmotic milieu and can be isolated from the homogenate by various approaches. Each enrichment method has its own benefits and drawbacks and there is not a single method that is optimal for all research purposes...Continue Reading

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Citations

May 3, 2021·Molecular & Cellular Proteomics : MCP·Charlotte A G H van Gelder, Maarten Altelaar
Jul 13, 2021·Frontiers in Cellular Neuroscience·Andres Di PaoloJosé Roberto Sotelo-Silveira

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Methods Mentioned

BETA
affinity purification
biopsies
density
membrane filtration
electrophoresis
electron microscopy

Software Mentioned

TMHMM
Hystar
GO
InteractiVenn
Mascot
TermFinder
Compass DataAnalysis
ImageJ
SynaptomeDB
SynGO geneset

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