Proteomic comparison of needles from blister rust-resistant and susceptible Pinus strobus seedlings reveals upregulation of putative disease resistance proteins

Molecular Plant-microbe Interactions : MPMI
Jason A SmithJeffrey H Gillman

Abstract

In order to characterize a hypersensitive-like reaction in selected Pinus strobus seedlings to Cronartium ribicola, a proteomic comparison of needles from resistant and susceptible seedlings was undertaken using two-dimensional gel electrophoresis (2-DE). The results revealed 19 polypeptides specific to resistant seedlings and seven of these specific to infected resistant seedlings. There were 13 polypeptides up-regulated (> or = 3-fold increase) in resistant family P327 in comparison to needle tissue from susceptible and mock-inoculated seedlings. Electrospray ionization liquid chromatography and tandem mass spectrometry was used to sequence 11 proteins from the 2-DE gels. Sequences obtained from electrospray ionization liquid chromatography and tandem mass spectrometry were used for MS-BLAST and Pro-ID database searches allowing identification with a 95 to 99% confidence level. Six proteins were determined to be homologs of proteins with known roles in disease resistance, five were determined to be homologs of members of the leucine-rich repeat (LRR) superfamily, and one was a homolog of heat shock protein 90, a protein that serves as a cofactor for certain LRR proteins. This is the first report of members of the LRR family w...Continue Reading

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Citations

Jul 31, 2013·Journal of Proteomics·Robert NawrotWaltraud X Schulze
Apr 9, 2013·Journal of Proteomics·Mark V BoggessKumar Bala
Jun 23, 2006·Proteomics·Neerav D Padliya, Bret Cooper
Jul 1, 2010·Journal of Biomedicine & Biotechnology·Raquel González-FernándezJesús V Jorrín-Novo
Nov 25, 2006·Molecular & Cellular Proteomics : MCP·William J SnellingColm J Lowery
Sep 10, 2019·Mass Spectrometry Reviews·Ana Margarida RodriguesCarla António
Feb 7, 2021·International Journal of Molecular Sciences·Vilnis Šķipars, Dainis Ruņģis
Mar 13, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Şeküre Çulha ErdalYasemin Ekmekçi

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