Proteomic comparison of selective breeding and growth hormone transgenesis in fish: Unique pathways to enhanced growth

Journal of Proteomics
Dwight R CauseyDaniel J Macqueen

Abstract

In fish used for food production and scientific research, fast growth can be achieved via selective breeding or induced instantaneously via growth hormone (GH) transgenesis (GHT). The proteomic basis for these distinct routes towards a similar higher phenotype remains uncharacterized, as are associated implications for health parameters. We addressed this knowledge gap using skeletal muscle proteomics in coho salmon (Oncorhynchus kisutch), hypothesising that i) selective breeding and GHT are underpinned by both parallel and unique changes in growth systems, and ii) rapidly-growing fish strains have lowered scope to allocate resources towards immune function. Quantitative profiling of GHT and growth-selected strains was done in comparison to wild-type after injection with PBS (control) or Poly I:C (to mimic infection). We identified remodelling of the muscle proteome in each growth-enhanced strain that was strikingly non-overlapping. GHT was characterized by focal upregulation of systems driving protein synthesis, while the growth-selected fish presented a larger and more diverse set of changes, consistent with complex alterations to many metabolic and cellular pathways. Poly I:C had little detectable effect on the muscle proteo...Continue Reading

Datasets Mentioned

BETA
PXD009537

Methods Mentioned

BETA
transgenic
acetylation
protein folding

Software Mentioned

R
missForest
BLASTp
MaxQuant
seriation
PERMANOVA
Andromeda
limma
UCSF Chimera
gplots

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