Proteomic identification of alterations in metabolic enzymes and signaling proteins in hypokalemic nephropathy

Proteomics
Visith ThongboonkerdPrida Malasit

Abstract

Hypokalemic nephropathy caused by prolonged K(+) deficiency is associated with metabolic alkalosis, polydipsia, polyuria, growth retardation, hypertension, and progressive tubulointerstitial injury. Its pathophysiology, however, remains unclear. We performed gel-based, differential proteomics analysis of kidneys from BALB/c mice fed with high-normal-K(+) (HNK), low-normal-K(+) (LNK), or K(+)-depleted diet for 8 wk (n = 6 in each group). Plasma K(+) levels were 4.62 +/- 0.35, 4.46 +/- 0.23, and 1.51 +/- 0.21 mmol/L for HNK, LNK, and KD mice, respectively (p < 0.0001; KD vs. others). With comparable amounts of food intake, the KD mice drank significantly more water than the other two groups and had polyuria. Additionally, the KD mice had growth retardation, metabolic alkalosis, markedly enlarged kidneys, renal tubular dilation, intratubular deposition of amorphous and laminated hyaline materials, and tubular atrophy. A total of 33 renal proteins were differentially expressed between the KD mice and others, whereas only eight proteins were differentially expressed between the HNK and LNK groups, as determined by quantitative intensity analysis and ANOVA with Tukey's post hoc multiple comparisons. Using MALDI-MS and/or quadrupole-T...Continue Reading

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Citations

Jun 21, 2011·Acta Pharmacologica Sinica·Hong LuLi-ying Zhu
May 23, 2012·Antioxidants & Redox Signaling·David SheehanBrian McDonagh
Jul 26, 2008·PLoS Computational Biology·Wannipha TongsimaPrasit Palittapongarnpim
Nov 4, 2010·Seminars in Nephrology·Ana VelicCarsten A Wagner
Mar 16, 2016·Biochimica Et Biophysica Acta·Paleerath PeerapenVisith Thongboonkerd
Aug 1, 2009·Proteomics. Clinical Applications·Hermann Lage
May 1, 2008·Proteomics. Clinical Applications·Nicolas BousetteThomas Kislinger
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Apr 25, 2014·American Journal of Physiology. Renal Physiology·Lene StødkildeJørgen Frøkiaer
Apr 10, 2009·Journal of Proteome Research·Phattara-Orn Havanapan, Visith Thongboonkerd
Nov 6, 2007·Journal of Proteome Research·Sa-Nga PattanakitsakulVisith Thongboonkerd

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