Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants.

Scientific Reports
Young Hwa JungHan-Suk Kim

Abstract

The immature preterm kidney is likely to be vulnerable to acute kidney injury (AKI). However, the biomarkers currently used for AKI are not sensitive or specific and are also inadequate for the timely detection of AKI in preterm infants. The objectives of this study were to identify novel urinary biomarkers of AKI using proteomic techniques, and to verify and validate that the candidates can serve as early predictive biomarkers for AKI. In total, 1,810 proteins were identified in the discovery phase. Among those proteins, 174 were selected as the 1st targeted proteins. A total of 168 proteins were quantified, and the levels of 6 were significantly increased in the AKI group in the verification phase. Using a clinical assay, the results were confirmed and validated using samples of the first urine after birth from the biorepository. Finally, enzyme-linked immunosorbent assays revealed that the levels of annexin A5, neutrophil gelatinase-associated lipocalin (NGAL), and protein S100-P were significantly higher in the samples of the first urine from patients with AKI than in those from patients without AKI. In conclusion, urinary annexin A5, NGAL and protein S100-P levels are promising biomarkers for early, accurate prediction of ...Continue Reading

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Citations

Mar 8, 2021·Jornal de pediatria·Robert H Mak, Carolyn L Abitbol
Jul 3, 2021·Frontiers in Medicine·Celien Kuiper-MakrisMiguel A Alejandre Alcazar
Jul 1, 2021·Expert Review of Proteomics·Qi Wu, Robert A Fenton

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISAs
ELISA
urine sample collection

Clinical Trials Mentioned

NCT02743273

Software Mentioned

Andromeda
SPSS
GTRMS
Spectronaut
MaxQuant
mProphet

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