Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 Nuclear Bodies.

BMC Cell Biology
Prabhakar RajanDavid J Elliott

Abstract

Active pre-mRNA splicing occurs co-transcriptionally, and takes place throughout the nucleoplasm of eukaryotic cells. Splicing decisions are controlled by networks of nuclear RNA-binding proteins and their target sequences, sometimes in response to signalling pathways. Sam68 (Src-associated in mitosis 68 kDa) is the prototypic member of the STAR (Signal Transduction and Activation of RNA) family of RNA-binding proteins, which regulate splicing in response to signalling cascades. Nuclear Sam68 protein is concentrated within subnuclear organelles called SLM/Sam68 Nuclear Bodies (SNBs), which also contain some other splicing regulators, signalling components and nucleic acids. We used proteomics to search for the major interacting protein partners of nuclear Sam68. In addition to Sam68 itself and known Sam68-associated proteins (heterogeneous nuclear ribonucleoproteins hnRNP A1, A2/B1 and G), we identified hnRNP L as a novel Sam68-interacting protein partner. hnRNP L protein was predominantly present within small nuclear protein complexes approximating to the expected size of monomers and dimers, and was quantitatively associated with nucleic acids. hnRNP L spatially co-localised with Sam68 as a novel component of SNBs and was als...Continue Reading

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Citations

May 14, 2011·Endocrine-related Cancer·Pamela BielliClaudio Sette
Nov 30, 2013·International Journal of Molecular Sciences·Flora Sánchez-Jiménez, Víctor Sánchez-Margalet
Jan 29, 2014·The Journal of Cell Biology·Takatoshi IijimaPeter Scheiffele
Feb 1, 2011·Proteomics·Xuefeng FangBiaoyang Lin
Aug 24, 2010·The Journal of Pathology·David J Elliott, Prabhakar Rajan
Nov 20, 2015·BMC Research Notes·Tangeng MaChristian R Gomez
Aug 15, 2015·BioMed Research International·Paola FrisoneMaria Paola Paronetto
Jul 6, 2016·The Journal of Cell Biology·Taro MannenTetsuro Hirose
Apr 7, 2017·Human Genetics·Jennifer MunkleyDavid J Elliott
Apr 6, 2021·Molecular Genetics & Genomic Medicine·Nasser Pouladi, Sepehr Abdolahi

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Methods Mentioned

BETA
immunoprecipitation
immunoprecipitations
two-hybrid
two hybrid
transfection
FCS
transfections

Software Mentioned

MASCOT
MOWSE ( Molecular Weight Search )

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