Proteomic identification of Syzygium cumini seed extracts by MALDI-TOF/MS

Applied Biochemistry and Biotechnology
Kumari BinitaSavita Yadav

Abstract

Syzygium cumini is traditionally used medicinal plant. The different part of the plant such as bark, leaves, seed and fruits are widely used as an alternative medicine in various diseases. Although the scientific community has a strong interest on S. cumini seed biochemistry focusing on metabolite composition, proteins have not yet been investigated. In the present study, we have applied a proteomic approach to study the proteome of the S. cumini seed using phenol extraction method for protein isolation, which were never analysed before. Fifteen brightly silver stained protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry after resolving on two-dimensional gel electrophoresis. These proteins have been found to involve in various functions such as antifungal, sulphur metabolism, carbohydrate metabolism, fruit ripening and softening, dormancy breaking and seed germination, hormone signalling, secondary metabolite transport, defence and stress response, nitrogen metabolism, synthesis and stabilization. Amongst the identified protein, lactoferrin was a mammalian origin protein with high nutritious and pharmaceutical value, which was purified by different types of chromatograph...Continue Reading

References

Feb 3, 1999·Annual Review of Genetics·P R Johnson, J R Ecker
Mar 23, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M T PepatoI L Brunetti
Oct 18, 2001·Journal of the American College of Nutrition·O M Conneely
Oct 25, 2001·Antiviral Research·B W van der StrateD K Meijer
Nov 16, 2001·Journal of Experimental Botany·M GijzenJ T Chernys
Oct 1, 1995·Plant Physiology·R. D. De CastroR. J. Bino
Nov 30, 2002·Phytotherapy Research : PTR·S S RathiN R Biswas
Mar 22, 2003·Journal of Ethnopharmacology·P Stanely Mainzen PrinceVenugopal P Menon
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Miguel A BallicoraJack Preiss
Mar 24, 2004·Journal of Ethnopharmacology·M Chandrasekaran, V Venkatesalu
Mar 12, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S B SridharM S Shastri
Apr 30, 2005·Current Opinion in Plant Biology·Kazufumi Yazaki
Jun 21, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kasiappan RaviSorimuthu Subramanian
Oct 18, 2005·Photosynthesis Research·Miguel A BallicoraJack Preiss
Oct 28, 2005·Gene Therapy·N Darbinian-SarkissianK Khalili
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Koji YamauchiMitsunori Takase
Feb 1, 2007·Annual Review of Plant Biology·Philip A Rea
Apr 5, 2007·Nature Protocols·Tal IsaacsonJocelyn K C Rose
May 14, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Bhavna SharmaPartha Roy
Apr 4, 2009·Journal of Cellular Biochemistry·Nune DarbinianShohreh Amini
Jul 9, 2009·International Review of Cell and Molecular Biology·Kazufumi YazakiKojiro Takanashi
Jul 9, 2009·The Journal of Clinical Endocrinology and Metabolism·J M Moreno-NavarreteJ M Fernández-Real
Oct 15, 2009·Plant Signaling & Behavior·Narendra Tuteja
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Joohyun KangYoungsook Lee
Mar 30, 2010·Journal of Proteome Research·Ramesh KatamTibor Pechan
Dec 1, 2010·Journal of Cellular Biochemistry·Nune DarbinianShohreh Amini
Jul 19, 2011·Virology Journal·Thomas CherrierChristian Schwartz
Jan 3, 2012·International Journal of Biological Macromolecules·Sanjay KumarSavita Yadav

❮ Previous
Next ❯

Citations

Mar 30, 2016·Applied Biochemistry and Biotechnology·Ibrahim O AlanaziAfshan Masood
Sep 16, 2015·Applied Biochemistry and Biotechnology·Zhe WangXinle Liang
Aug 1, 2015·Omics : a Journal of Integrative Biology·Nilay Budeyri Gokgoz, Berna Sariyar Akbulut
Feb 13, 2015·Applied Biochemistry and Biotechnology·Neha KumariSavita Yadav

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

© 2021 Meta ULC. All rights reserved