Proteomic identification of the differentially expressed proteins in human lung epithelial cells by airborne particulate matter

Journal of Applied Toxicology : JAT
Yu Mi JeonMi-Young Lee

Abstract

Exposure to airborne PM₁₀, particulate matter with a median aerodynamic diameter of less than 10 µm, is known to be associated with a number of adverse health effects. To gain a better understanding of the cytotoxic mechanism and to develop protein biomarker candidates for PM₁₀-induced toxicity, proteomic analyses were performed in human lung epithelial cells. Two-dimensional gel electrophoresis (2-DE) was followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to analyze the proteins differentially expressed by exposure to PM₁₀. Analysis of 2-DE gels revealed more than 1270 protein spots in the cells, of which 36 showed changes of more than 2-fold on exposure to PM₁₀ (up-regulation, n = 6; down-regulation, n = 30). The glycolytic enzyme pyruvate kinase, which also plays a role in tumor metabolism, showed a marked increase in expression, whereas the cytoskeleton-related vinculin and anti-inflammatory annexin 1 showed marked decreases in expression.

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Citations

Nov 2, 2011·Biochimica Et Biophysica Acta·Seul-Ki ParkMi-Young Lee
May 6, 2011·Biomaterials·Jasmine J LiLin-Yue Lanry Yung
Apr 15, 2011·Journal of Applied Toxicology : JAT·Seul Ki ParkMi Young Lee
Aug 15, 2013·Journal of Applied Toxicology : JAT·Qingyu HuangHeqing Shen
Apr 16, 2016·Journal of Applied Toxicology : JAT·Yu-Mi Jeon, Mi-Young Lee
Apr 7, 2017·Journal of Cancer Prevention·Hyo Jeong KimYoung Rok Seo

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