Proteomic identification of the silkworm (Bombyx mori L) prothoracic glands during the fifth instar stage

Bioscience Reports
Xiao-feng WuYun-gen Miao

Abstract

Although the ecdysteroid of the silkworm had been studied for decades, the proteome of the prothoracic gland, the primary source of ecdysteroid hormones, has not been studied previously. In the present paper, we utilized a proteomic approach to investigate the fifth instar prothoracic gland during the growth and development of the silkworm, Bombyx mori L. The two-dimensional electrophoresis results showed that the majority of proteins were acidic proteins, especially concentrated in the area of 25-65 kDa, with pI values of between 4 and 7, and the difference was not distinct. When compared with Qiufeng (Japanese strain), the interspecific distinction was larger than the intraspecific distinction, and 19 particular spots, excized from the third, fifth and ninth days of p50 (Chinese strain) and Qiufeng were subjected to MALDI-TOF-MS (matrix-assisted laser-desorption ionization-time-of-flight MS) analysis. We sorted them into seven catagories: energetics and/or metabolism, storage proteins, protection, lipid metabolism, signal transduction, cell function and unknown function proteins. Of these proteins, arginine methyltransferase is discussed as playing an important role in regulating the activation of ecdysteroidogenesis via tran...Continue Reading

References

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Oct 7, 2006·Journal of Proteome Research·Xing-hua LiYun-gen Miao

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Citations

Nov 24, 2012·Journal of Proteomics·Xiao-Feng ShiZhi-Fang Zhang
May 5, 2012·Photochemistry and Photobiology·Caroline SolazzoPaul Wyeth
Apr 2, 2016·Biochimica Et Biophysica Acta·Venugopal Reddy BovillaAnitha Mamillapalli

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