Proteomic Insights into Starvation of Nitrogen-Replete Cells of Nostoc sp. PCC 7120 under β-N-Methylamino-L-Alanine (BMAA) Treatment.

Toxins
Olga A KoksharovaVadim M Govorun

Abstract

All cyanobacteria produce a neurotoxic non-protein amino acid β-N-methylamino-L-alanine (BMAA). However, the biological function of BMAA in the regulation of cyanobacteria metabolism still remains undetermined. It is known that BMAA suppresses the formation of heterocysts in diazotrophic cyanobacteria under nitrogen starvation conditions, and BMAA induces the formation of heterocyst-like cells under nitrogen excess conditions, by causing the expression of heterocyst-specific genes that are usually "silent" under nitrogen-replete conditions, as if these bacteria receive a nitrogen deficiency intracellular molecular signal. In order to find out the molecular mechanisms underlying this unexpected BMAA effect, we studied the proteome of cyanobacterium Nostoc sp. PCC 7120 grown under BMAA treatment in nitrogen-replete medium. Experiments were performed in two experimental settings: (1) in control samples consisted of cells grown without the BMAA treatment and (2) the treated samples consisted of cells grown with addition of an aqueous solution of BMAA (20 µM). In total, 1567 different proteins of Nostoc sp. PCC 7120 were identified by LC-MS/MS spectrometry. Among them, 80 proteins belonging to different functional categories were ch...Continue Reading

References

Jan 1, 1973·Advances in Enzymology and Related Areas of Molecular Biology·J M Buchanan
Jan 3, 2001·Journal of Bacteriology·A HerreroE Flores
Aug 2, 2001·The Journal of Biological Chemistry·M I Muro-PastorF J Florencio
Dec 12, 2002·Chemical Reviews·Lizbeth Hedstrom
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marika Lindahl, Francisco J Florencio
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Tansy ChiaAlison M Smith
Aug 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan J MurchSandra Anne Banack
Sep 10, 2004·Acta Neurologica Scandinavica·S J MurchO W Sacks
Apr 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul Alan CoxBirgitta Bergman
Sep 5, 2006·Cellular and Molecular Life Sciences : CMLS·X-B QiuL Wang
Nov 24, 2006·The Journal of Biological Chemistry·Nicole M KoropatkinThomas J Smith
Jan 10, 2008·Trends in Microbiology·Karl Forchhammer
Jan 13, 2009·Trends in Biochemical Sciences·Isabella Dalle-DonneAldo Milzani
Mar 17, 2009·Toxicon : Official Journal of the International Society on Toxinology·Peter B Nunn, Malarvizhi Ponnusamy
Dec 16, 2009·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Peter B Nunn
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sara JonassonBirgitta Bergman
Jun 28, 2011·Toxicon : Official Journal of the International Society on Toxinology·S DowningT G Downing
Jul 31, 2013·Environment International·Sylvain MerelOlivier Thomas
Aug 24, 2013·Marine Drugs·Lotta BerntzonBirgitta Bergman
Jan 7, 2014·PloS One·Liying JiangUlla Rasmussen
Oct 12, 2014·Chemico-biological Interactions·Trevor M Penning
Jun 2, 2015·Research in Microbiology·Katharina Besemer
Oct 2, 2015·Microbiology and Molecular Biology Reviews : MMBR·Luciano F Huergo, Ray Dixon
Nov 4, 2015·The FEBS Journal·Karl Forchhammer, Jan Lüddecke
Mar 8, 2016·Toxicon : Official Journal of the International Society on Toxinology·Simoné Downing, Timothy Grant Downing
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·A A Popova, O A Koksharova
Nov 25, 2016·Frontiers in Microbiology·Corinne Cassier-ChauvatFranck Chauvat
Feb 17, 2017·FEMS Microbiology Reviews·Antonia HerreroEnrique Flores

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence resonance
Protein Assay

Software Mentioned

ProteinPilot
MaxQuant

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.