Proteomic Investigation to Identify Anticancer Targets of Nemopilema nomurai Jellyfish Venom in Human Hepatocarcinoma HepG2 Cells

Toxins
Indu ChoudharyEuikyung Kim

Abstract

Nemopilema nomurai is a giant jellyfish that blooms in East Asian seas. Recently, N. nomurai venom (NnV) was characterized from a toxicological and pharmacological point of view. A mild dose of NnV inhibits the growth of various kinds of cancer cells, mainly hepatic cancer cells. The present study aims to identify the potential therapeutic targets and mechanism of NnV in the growth inhibition of cancer cells. Human hepatocellular carcinoma (HepG2) cells were treated with NnV, and its proteome was analyzed using two-dimensional gel electrophoresis, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF/MS). The quantity of twenty four proteins in NnV-treated HepG2 cells varied compared to non-treated control cells. Among them, the amounts of fourteen proteins decreased and ten proteins showed elevated levels. We also found that the amounts of several cancer biomarkers and oncoproteins, which usually increase in various types of cancer cells, decreased after NnV treatment. The representative proteins included proliferating cell nuclear antigen (PCNA), glucose-regulated protein 78 (GRP78), glucose-6-phosphate dehydrogenase (G6PD), elongation factor 1γ (EF1γ), nucleolar and s...Continue Reading

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Apr 25, 2020·Frontiers in Molecular Biosciences·Boris Negrutskii
Feb 14, 2021·International Journal of Biological Macromolecules·S Amreen NisaRahul Vivek
Jun 16, 2021·Journal of Medicinal Chemistry·Xianghai RenQun Qian

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Methods Mentioned

BETA
electrophoresis
protein folding
xenograft
antisense oligonucleotide
Assay

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STRING ( Search Tool for the Retrieval of Interacting Genes / ...
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