Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca²⁺ influx

Nature Cell Biology
Ji JingYubin Zhou

Abstract

Specialized junctional sites that connect the plasma membrane (PM) and endoplasmic reticulum (ER) play critical roles in controlling lipid metabolism and Ca(2+) signalling. Store-operated Ca(2+) entry mediated by dynamic STIM1-ORAI1 coupling represents a classical molecular event occurring at ER-PM junctions, but the protein composition and how previously unrecognized protein regulators facilitate this process remain ill-defined. Using a combination of spatially restricted biotin labelling in situ coupled with mass spectrometry and a secondary screen based on bimolecular fluorescence complementation, we mapped the proteome of intact ER-PM junctions in living cells without disrupting their architectural integrity. Our approaches led to the discovery of an ER-resident multi-transmembrane protein that we call STIMATE (STIM-activating enhancer, encoded by TMEM110) as a positive regulator of Ca(2+) influx in vertebrates. STIMATE physically interacts with STIM1 to promote STIM1 conformational switch. Genetic depletion of STIMATE substantially reduces STIM1 puncta formation at ER-PM junctions and suppresses the Ca(2+)-NFAT signalling. Our findings enable further genetic studies to elucidate the function of STIMATE in normal physiology...Continue Reading

References

Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Mayer, M Bendayan
Mar 15, 2000·Journal of Molecular Biology·W P Russ, D M Engelman
Mar 10, 2001·Science·M Bendayan
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner
Mar 25, 1957·The Journal of Biophysical and Biochemical Cytology·K R PORTER, G E PALADE
Jun 11, 2004·Biophysical Journal·Tomasz Zal, Nicholas R J Gascoigne
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shenyuan L ZhangMichael D Cahalan
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sang-Won MinThomas C Südhof
Apr 5, 2007·Nature Protocols·Holger LorenzJennifer Lippincott-Schwartz
Feb 6, 2008·Current Biology : CB·Ilya GrigorievAnna Akhmanova
Feb 3, 2009·Nature Cell Biology·Joseph P YuanShmuel Muallem
Apr 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Youjun WangDonald L Gill
Dec 29, 2009·Nature Structural & Molecular Biology·Yubin ZhouPatrick G Hogan
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yubin ZhouPatrick G Hogan
Mar 24, 2010·Annual Review of Immunology·Patrick G HoganAnjana Rao
May 10, 2011·Annual Review of Biochemistry·Silvia Carrasco, Tobias Meyer
Oct 1, 2011·Trends in Biochemical Sciences·Yael Elbaz, Maya Schuldiner
Aug 24, 2012·Nature Reviews. Molecular Cell Biology·Jonathan SoboloffDonald L Gill
Jan 5, 2013·Science·Le CongFeng Zhang
Mar 26, 2013·Current Opinion in Cell Biology·Christopher J StefanScott D Emr
Jul 16, 2013·Nature Structural & Molecular Biology·Yubin ZhouPatrick G Hogan
Feb 5, 2014·Nature Communications·Xizhuo WangDonald L Gill
Nov 25, 2014·Nature Methods·Stephanie S LamAlice Y Ting
Jul 18, 2015·Nature Communications·Guolin MaYubin Zhou

❮ Previous
Next ❯

Citations

Mar 1, 2016·Current Opinion in Cell Biology·Tim P Levine, Sandip Patel
Mar 1, 2016·Biochimica Et Biophysica Acta·Chi-Lun Chang, Jen Liou
May 27, 2016·Nature Communications·Bipan Kumar DebGaiti Hasan
Jul 15, 2016·Channels·Letizia AlbarranJuan A Rosado
Oct 25, 2016·Trends in Cell Biology·Dae In Kim, Kyle J Roux
Nov 24, 2016·International Journal of Molecular Sciences·Jong-Mok Lee, Satoru Noguchi
Feb 6, 2017·Journal of the American Chemical Society·Song-Yi LeeHyun-Woo Rhee
Apr 8, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Chiao-Lin Chen, Norbert Perrimon
Dec 27, 2015·Biochimica Et Biophysica Acta·Markus Hoth
Jun 8, 2017·Biochemistry·Qian ChuAlan Saghatelian
Dec 9, 2016·Nature Communications·Yandong ZhouDonald L Gill
May 31, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Chi-Lun ChangJen Liou
Jul 21, 2017·Journal of Cell Science·Marek K KorzeniowskiTamas Balla
Oct 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anoop Singh ChauhanBoyi Gan
Apr 2, 2019·Médecine sciences : M/S·Benoît BégantonJérôme Solassol
Apr 11, 2018·Analytical and Bioanalytical Chemistry·Jiaqi FuXiaoyun Liu
Oct 1, 2015·Nature Cell Biology·Robert Hooper, Jonathan Soboloff
May 24, 2019·The Journal of Physiology·Ji JingYubin Zhou
Jul 5, 2019·Expert Review of Proteomics·Benoît BégantonJérôme Solassol
Jan 31, 2016·American Journal of Physiology. Cell Physiology·Isabella DerlerChristoph Romanin
Aug 11, 2017·Nature Protocols·Jeffrey D MartellAlice Y Ting
Sep 27, 2019·Current Drug Targets·Sampath Bhuvaneshwari, Kavitha Sankaranarayanan
Sep 11, 2019·Cold Spring Harbor Perspectives in Biology·Hwei Ling Ong, Indu Suresh Ambudkar
Oct 23, 2019·Cold Spring Harbor Perspectives in Biology·Malini AhujaShmuel Muallem
Oct 2, 2019·Cold Spring Harbor Perspectives in Biology·Richard S Lewis
Jan 8, 2016·American Journal of Physiology. Cell Physiology·Aran SonShmuel Muallem
Mar 17, 2017·Annual Review of Biochemistry·Yasunori Saheki, Pietro De Camilli
Aug 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nityanand SrivastavaDolly Mehta
Jun 9, 2016·The Biochemical Journal·Jiwon Hwang, Peter J Espenshade
Jun 11, 2017·The Journal of Cell Biology·Yu-Ju ChenJen Liou
May 3, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Namju KangDong Min Shin
Jan 1, 2017·Current Molecular Biology Reports·Sven KappelAnna Borgström
Mar 27, 2020·Frontiers in Oncology·Zhengfeng YangZhenyao Xu

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Methods Mentioned

BETA
dissection
bimolecular fluorescence complementation
FRET
electron microscopy
coimmunoprecipitation
pull down
immunoprecipitation
PCR
pulldown
electrophoresis

Software Mentioned

STIMATE
FlowJo
NIKON NIS - Elements AR package
MetaFluor
LiMETER
Elements AR
ImageJ
NIS
Prism5
Matlab

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