Proteomic Methods of Detection and Quantification of Protein Toxins

Toxins
Miloslava DuracovaJiri Dresler

Abstract

Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to compare conventional detection methods of protein toxins such as in vitro bioassays with proteomic methods, including immunoassays and mass spectrometry-based techniques and their combination. Special emphasis is given to toxins falling into a group of selected agents, according to the Centers for Disease Control and Prevention, such as Staphylococcal enterotoxins, Bacillus anthracis toxins, Clostridium botulinum toxins, Clostridium perfringens epsilon toxin, ricin from Ricinus communis, Abrin from Abrus precatorius or control of trade in dual-use items in the European Union, including lesser known protein toxins such as Viscumin from Viscum album. The analysis of protein toxins and monitoring for biological threats, i.e., the deliberate spread of infectious microorganisms or toxins through water, food, or the air, requires rapid and reliable methods for the early identification of these agents.

References

Sep 1, 1977·Infection and Immunity·K E HejtmancikA Kurosky
Aug 1, 1979·Japanese Journal of Medical Science & Biology·S KozakiS Notermans
Apr 1, 1975·Seminars in Nuclear Medicine·S J Goldsmith
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C C HarrisI C Hsu
Nov 1, 1977·Applied and Environmental Microbiology·G C Saunders, M L Bartlett
Feb 1, 1978·Japanese Journal of Medical Science & Biology·S NotermansM Schothorst
Dec 1, 1990·International Journal of Food Microbiology·M TakahashiG Sakaguchi
Oct 1, 1989·The Journal of Infectious Diseases·L H HarrisonA F Kaufmann
Aug 21, 1986·Journal of Immunological Methods·I F HahnH Brandis
Feb 1, 1985·Journal of Clinical Microbiology·M Dezfulian, J G Bartlett
Mar 1, 1985·Diagnostic Microbiology and Infectious Disease·M Dezfulian, J G Bartlett
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·A C AshtonJ O Dolly
Nov 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·L L Salomon, R W Tew
Feb 1, 1984·Journal of Clinical Microbiology·B A McClane, R J Strouse
Dec 1, 1981·Applied and Environmental Microbiology·G E LewisJ F Metzger
Sep 1, 1981·Journal of Toxicology and Environmental Health·A GodalA Pihl
Apr 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·M AdlerS S Deshpande
Apr 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·S S DeshpandeM Adler
Nov 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·M A PoliG A Merrill
Sep 1, 1994·Toxicology and Applied Pharmacology·L B PearceR D MacCallum
Jan 7, 1998·Letters in Applied Microbiology·F A UzalW R Kelly

❮ Previous
Next ❯

Citations

Jul 20, 2019·Toxins·Robert J HobbsChristopher D Gwenin
Mar 27, 2019·Toxins·Miloslava DuracovaZuzana Krocova
Dec 1, 2019·Scientific Reports·Aashish Jain, Daisuke Kihara
Feb 12, 2021·Archives of Toxicology·Osnat RosenRan Zichel
Mar 31, 2021·Critical Reviews in Food Science and Nutrition·Karolinny Cristiny de Oliveira VieiraLizziane Kretli Winkelstroter Eller
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Poorvisha Ravi, Muthupandian Ganesan
Mar 13, 2019·Journal of the American Society for Mass Spectrometry·William R A WichertNicholas E Manicke

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assays
PCR
ELISA
electrophoresis
surface plasmon resonance
acetylation
chemical treatment
ELISAs
FRET
RIP

Software Mentioned

Skyline

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Related Papers

Clinical Biochemistry
Leroi V DeSouza, K W Michael Siu
Journal - Forensic Science Society
E G CLARKE, D J Humphreys
Clinical Laboratory Science : Journal of the American Society for Medical Technology
Lynda A Britton
© 2022 Meta ULC. All rights reserved